Journal article
Cloning and sequencing show that 4-hydroxybenzoate hydroxylase (PobA) is required for uptake of 4-hydroxybenzoate in Rhizobium leguminosarum
Microbiology, Vol.140(10), pp.2775-2786
1994
Abstract
Mutants of Rhizobium leguminosarum bv. viciae MNF300 and R. leguminosarum bv. trifolii WU95 unable to accumulate 4-hydroxybenzoate lack 4-hydroxybenzoate hydroxylase. The capacity of these mutants to take up and grow on 4-hydroxybenzoate was restored by a 2.0 kb EcoRI-PstI DNA fragment. This contained only one ORF which had over 60% DNA sequence similarity with the structural gene for 4-hydroxybenzoate hydroxylase (pobA) from Pseudomonas spp. and Acinetobacter. Reported effects of metabolic inhibitors and substrate analogues on the apparent uptake of 4-hydroxybenzoate have now been shown to be due to their direct effect on 4-hydroxybenzoate hydroxylase. We propose that uptake of 4-hydroxybenzoate is via a metabolic 'drag' mechanism dependent on the activity of the pobA gene product.
Details
- Title
- Cloning and sequencing show that 4-hydroxybenzoate hydroxylase (PobA) is required for uptake of 4-hydroxybenzoate in Rhizobium leguminosarum
- Authors/Creators
- C.M. Wong (Author/Creator) - Murdoch UniversityM.J. Dilworth (Author/Creator)A.R. Glenn (Author/Creator)
- Publication Details
- Microbiology, Vol.140(10), pp.2775-2786
- Publisher
- Society for General Microbiology
- Identifiers
- 991005541807007891
- Murdoch Affiliation
- School of Biological and Environmental Sciences
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.83 Bioengineering
- 3.83.323 Bioremediation
- Web Of Science research areas
- Microbiology
- ESI research areas
- Microbiology