Journal article
Clostridioides (Clostridium) difficile in children with diarrhoea in Vietnam
Anaerobe, Vol.74, Art. 102550
2022
Abstract
Background
Clostridioides (Clostridium) difficile commonly causes hospital-acquired infection which can range from mild diarrhoea to life-threatening toxic megacolon and even death. Reports on C. difficile infection (CDI) in Vietnam are limited, so this study was designed to evaluate the prevalence, molecular epidemiology and antimicrobial susceptibility of C. difficile isolated from children with diarrhoea in Vietnam. Infants are often colonised with C. difficile and it was hypothesised that those colonising strains would represent strains of C. difficile circulating in the hospital/region at the time, however, this was not an attempt to determine if C. difficile was the cause of the diarrhoea.
Methods
Diarrhoeal stool samples collected at two children's hospitals in northern Vietnam from October 1, 2020 to February 28, 2021 were transported to Perth, Western Australia, for culture of C. difficile and further investigations on isolates; PCR ribotyping, toxin gene profiling and antimicrobial susceptibility testing.
Results
From these hospitals, 370 diarrhoeal stool samples were collected, most from children aged 1–15 months (71.9%; 266/370). The overall prevalence of C. difficile in stool samples from children aged ≤16 years was 37.8% (140/370) and the highest prevalence was in the 2–12 months age group (52.9%; 74/140). In total, 151 isolates of C. difficile were recovered; the proportion of toxigenic isolates was 16.6% (25/151). Of the 25 toxigenic C. difficile isolates, the toxin gene profiles A+B+CDT− and A−B+CDT− comprised 72% and 28%, respectively. The four most prevalent C. difficile ribotypes (RTs) were QX 011 (25/151), RT 010 (25/151), QX 107 (12/151) and RT 012 (11/151). All isolates were susceptible to vancomycin, metronidazole and fidaxomicin, while there was significant resistance to clindamycin (90.1%), and some to moxifloxacin (6.6%) and rifaximin (3.3%).
Conclusion
The prevalence of C. difficile in children with diarrhoea was high (37.8%) although the proportion of toxigenic strains was comparatively low. The clinical significance of any isolate needs to be determined.
Details
- Title
- Clostridioides (Clostridium) difficile in children with diarrhoea in Vietnam
- Authors/Creators
- P.A. Khun (Author/Creator) - The University of Western AustraliaL.D. Phi (Author/Creator) - Thai Binh University of Medicine and PharmacyP.T. Pham (Author/Creator) - Thai Binh Paediatric Hospital, Thai Binh, Viet NamH.T. Thu Nguyen (Author/Creator)Q.T. Huyen Vu (Author/Creator)D.A. Collins (Author/Creator) - Edith Cowan UniversityT.V. Riley (Author/Creator) - Murdoch University
- Publication Details
- Anaerobe, Vol.74, Art. 102550
- Publisher
- Academic Press
- Identifiers
- 991005544524207891
- Copyright
- © 2022 Elsevier Ltd.
- Murdoch Affiliation
- School of Medical, Molecular and Forensic Sciences
- Language
- English
- Resource Type
- Journal article
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- 1 Clinical & Life Sciences
- 1.120 Inflammatory Bowel Diseases & Infections
- 1.120.1133 Clostridium Infections
- Web Of Science research areas
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