Co-infection with three mycoviruses stimulates growth of a Monilinia fructicola isolate on nutrient medium, but does not induce hypervirulence in a natural host

T.T. Tran; H. Li; D.Q. Nguyen; M.G.K. Jones; S.J. Wylie

doi:10.3390/v11010089

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Co-infection with three mycoviruses stimulates growth of a Monilinia fructicola isolate on nutrient medium, but does not induce hypervirulence in a natural host

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Co-infection with three mycoviruses stimulates growth of a Monilinia fructicola isolate on nutrient medium, but does not induce hypervirulence in a natural host

T.T. Tran, H. Li, D.Q. Nguyen, M.G.K. Jones and S.J. Wylie

Viruses, Vol.11(1), Article 89

2019

DOI: https://doi.org/10.3390/v11010089

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Abstract

Monilinia fructicola and Monilinia laxa are the most destructive fungal species infecting stone fruit (Prunus species). High-throughput cDNA sequencing of M. laxa and M. fructicola isolates collected from stone fruit orchards revealed that 14% of isolates were infected with one or more of three mycoviruses: Sclerotinia sclerotiorum hypovirus 2 (SsHV2, genus Hypovirus), Fusarium poae virus 1 (FPV1, genus Betapartitivirus), and Botrytis virus F (BVF, genus Mycoflexivirus). Isolate M196 of M. fructicola was co-infected with all three viruses, and this isolate was studied further. Several methods were applied to cure M196 of one or more mycoviruses. Of these treatments, hyphal tip culture either alone or in combination with antibiotic treatment generated isogenic lines free of one or more mycoviruses. When isogenic fungal lines were cultured on nutrient agar medium in vitro, the triple mycovirus-infected parent isolate M196 grew 10% faster than any of the virus-cured isogenic lines. BVF had a slight inhibitory effect on growth, and FPV1 did not influence growth. Surprisingly, after inoculation to fruits of sweet cherry, there were no significance differences in disease progression between isogenic lines, suggesting that these mycoviruses did not influence the virulence of M. fructicola on a natural host.

Details

Title: Co-infection with three mycoviruses stimulates growth of a Monilinia fructicola isolate on nutrient medium, but does not induce hypervirulence in a natural host
Authors/Creators: T.T. Tran (Author/Creator) - Murdoch University
H. Li (Author/Creator) - Murdoch University
D.Q. Nguyen (Author/Creator) - Murdoch University
M.G.K. Jones (Author/Creator) - Murdoch University
S.J. Wylie (Author/Creator) - Murdoch University
Publication Details: Viruses, Vol.11(1), Article 89
Publisher: MDPI
Identifiers: 991005541182807891
Copyright: © 2019 by the authors. Licensee MDPI, Basel, Switzerland.
Murdoch Affiliation: State Agricultural Biotechnology Centre; School of Veterinary and Life Sciences
Language: English
Resource Type: Journal article

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Citation topics: 3 Agriculture, Environment & Ecology; 3.267 Virology - Plant; 3.267.356 Plant Virus Interactions
Web Of Science research areas: Virology
ESI research areas: Microbiology