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Journal article
Collection of preputial material by scraping and aspiration for the diagnosis of Tritrichomonas foetus in bulls
Journal of the South African Veterinary Association, Vol.73(2), pp.66-69
2002
PMID: 12240772
Abstract
Two trials were carried out to assess the diagnostic sensitivity and practicability of preputial scraping as a method of collecting preputial material from bulls infected with Tritrichomonas foetus. In the 1st trial, preputial material was collected by simultaneous scraping and aspiration from 3 infected and 1 uninfected bull 10 times over a 5-week period. In the 2nd trial, samples from 5 infected bulls were collected by both sheath washing and scraping on 6 occasions, while 8 uninfected animals were sampled 3 times. Samples were cultured using a modified Trichomonas culture medium (Oxoid). In the first trial, 29 of 30 samples from infected bulls were found to be positive. In the second trial, 83 % of samples collected by both methods tested positive. In neither trial were any samples from the control bulls found to be positive. Scraping was found to be quick and safe, and offered advantages over preputial washing in that urine contamination was easily avoided, samples were smaller and more concentrated and contamination was reduced. It may, however, be subject to greater operator variability than sheath washing. It is concluded that preputial scraping is as effective as washing and represents a suitable alternative for the collection of material for direct examination and culture of Tritrichomonas foetus.
Details
- Title
- Collection of preputial material by scraping and aspiration for the diagnosis of Tritrichomonas foetus in bulls
- Authors/Creators
- Pete Charles Irons - Murdoch University, School of Veterinary MedicineM. M. Henton - Onderstepoort Veterinary Academic HospitalH.J. Bertschinger - University of Pretoria
- Publication Details
- Journal of the South African Veterinary Association, Vol.73(2), pp.66-69
- Publisher
- AOSIS Publishing
- Number of pages
- 4
- Identifiers
- 991005875553307891
- Copyright
- © 2002 P.C. Irons, M.M. Henton, H.J. Bertschinger
- Murdoch Affiliation
- School of Veterinary Medicine
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 1 Clinical & Life Sciences
- 1.248 Sexually Transmitted Infections
- 1.248.2104 Trichomonas Vaginalis
- Web Of Science research areas
- Veterinary Sciences
- ESI research areas
- Plant & Animal Science