Journal article
Colon cryptogenesis: Asymmetric budding
PloS one, Vol.8(10)
2013
Abstract
The process of crypt formation and the roles of Wnt and cell-cell adhesion signaling in cryptogenesis are not well described; but are important to the understanding of both normal and cancer colon crypt biology. A quantitative 3D-microscopy and image analysis technique is used to study the frequency, morphology and molecular topography associated with crypt formation. Measurements along the colon reveal the details of crypt formation and some key underlying biochemical signals regulating normal colon biology. Our measurements revealed an asymmetrical crypt budding process, contrary to the previously reported symmetrical fission of crypts. 3D immunofluorescence analyses reveals heterogeneity in the subcellular distribution of E-cadherin and β-catenin in distinct crypt populations. This heterogeneity was also found in asymmetrical budding crypts. Singular crypt formation (i.e. no multiple new crypts forming from one parent crypt) were observed in crypts isolated from the normal colon mucosa, suggestive of a singular constraint mechanism to prevent aberrant crypt production. The technique presented improves our understanding of cryptogenesis and suggests that excess colon crypt formation occurs when Wnt signaling is perturbed (e.g. by truncation of adenomatous polyposis coli, APC protein) in most colon cancers.
Details
- Title
- Colon cryptogenesis: Asymmetric budding
- Authors/Creators
- C.W. Tan (Author/Creator) - Walter and Eliza Hall Institute of Medical ResearchY. Hirokawa (Author/Creator) - The University of MelbourneB.S. Gardiner (Author/Creator) - Ludwig Institute for Cancer Research, Melbourne-Parkville Branch, Parkville, Victoria, AustraliaD.W. Smith (Author/Creator) - School of Computer Science and Software EngineeringA.W. Burgess (Author/Creator) - Walter and Eliza Hall Institute of Medical Research
- Publication Details
- PloS one, Vol.8(10)
- Publisher
- Public Library of Science
- Identifiers
- 991005540257307891
- Copyright
- © 2013 Tan et al.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 1 Clinical & Life Sciences
- 1.108 Molecular & Cell Biology - Cancer & Development
- 1.108.757 Wnt-Cadherin Interactions
- Web Of Science research areas
- Cell Biology
- ESI research areas
- Molecular Biology & Genetics