Colorimetric and electrophoretic evaluation of lipoprotein fractions in healthy neonatal calves: Comparison with results from adult cows and from calves with inflammatory conditions

A. Giordano; G. Rossi; M. Probo; P. Moretti; S. Paltrinieri

doi:10.1016/j.rvsc.2017.02.007

$Colorimetric and electrophoretic evaluation of lipoprotein fractions in healthy neonatal calves: Comparison with results from adult cows and from calves with inflammatory conditions$

Journal article

Open access

Peer reviewed

Colorimetric and electrophoretic evaluation of lipoprotein fractions in healthy neonatal calves: Comparison with results from adult cows and from calves with inflammatory conditions

A. Giordano, G. Rossi, M. Probo, P. Moretti and S. Paltrinieri

Research in Veterinary Science, Vol.111, pp.108-112

2017

DOI: https://doi.org/10.1016/j.rvsc.2017.02.007

Files and links (2)

pdf

colorimetric-and-electrophoretic-evaluation-of-lipoprotein-fractions.pdfDownload View

Author’s VersionCC BY-NC-ND V4.0, Open Access

url

Link to Published Version *Subscription may be requiredView

Abstract

High density lipoproteins (HDLs) are pivotal in innate immunity and decrease in serum during inflammation. Several studies have been done about lipoprotein changes in transition cows but little is known about their changes in newborn calves. The aim of this study is to provide information about HDLs in newborn calves, by defining the possible age-related changes in healthy calves compared with adults and by assessing the possible differences in calves with inflammation. Lipoprotein electrophoretic separation (reported as percentages) and colorimetric measurement of HDL (HDL-C) were performed on healthy cows and calves in order to identify possible differences in the lipoprotein profile due to the age. Then, age-matched calves with inflammatory conditions were also evaluated. Results showed that in calves HDL% and VLDL% were lower (mean values ± SD: 77.6% ± 8.6% and 2.6% ± 2.5%, respectively) and LDL% was higher (19.7% ± 7.4%) than in adults (89.0% ± 3.9%; 5.2 ± 2.1% and 5.8% ± 3.1%, respectively). Sick calves revealed a decrease of both HDL% (mean values ± SD: 61.0% ± 22.1%) and HDL-C (22.8 ± 11.6 mg/dL) and an increase of VLDL% (12.1% ± 13.1%) compared with controls (77.6% ± 8.6%; 41.5 ± 11.2 mg/dL and 2.6% ± 2.5%, respectively). Paraoxonase-1 activity, influenced by inflammation and oxidation, was measured, and it appeared correlated with HDL% and HDL-C in sick calves. In conclusion, this study revealed that HDLs concentration in healthy calves is lower than in adults, and further decreases in calves with inflammation, likely due to oxidation.

Details

Title: Colorimetric and electrophoretic evaluation of lipoprotein fractions in healthy neonatal calves: Comparison with results from adult cows and from calves with inflammatory conditions
Authors/Creators: A. Giordano (Author/Creator) - University of Milan
G. Rossi (Author/Creator) - University of Milan
M. Probo (Author/Creator) - University of Milan
P. Moretti (Author/Creator) - University of Milan
S. Paltrinieri (Author/Creator) - University of Milan
Publication Details: Research in Veterinary Science, Vol.111, pp.108-112
Publisher: Elsevier B.V.
Identifiers: 991005544913507891
Copyright: © 2017 Elsevier Ltd
Murdoch Affiliation: School of Veterinary and Life Sciences
Language: English
Resource Type: Journal article

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites

Metrics

104 File views/ downloads

31 Record Views

5 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.68 Lipids; 1.68.69 Lipoprotein Metabolism
Web Of Science research areas: Veterinary Sciences
ESI research areas: Plant & Animal Science