Journal article
Comparative Primary Metabolite Profiling of Setaria viridis Reveals Potential Markers to Water Limitation
Agriculture (Basel), Vol.13(3), 660
2023
Abstract
Growing varieties with higher water-use efficiency is crucial to address water limitation in agriculture. Breeding programs often resort to model plants, and Setaria viridis has been consolidating its position as a model for C-4 grasses. However, we lack a detailed analysis of drought-induced metabolic changes in S. viridis. To partially redress this, we assessed the primary metabolic profile of roots, leaves, and panicles in response to three watering levels. Five-day-old seedlings were submitted to water-limiting conditions for 25 days when samples were harvested. GC-MS-based analysis revealed that each plant organ had a specific metabolic profile, with TCA intermediates altered in above- and underground parts. The sPLS-DA analysis allowed clear separation of the water regimes for the three organs. Of the 36 most important metabolites, only four (sucrose, glycerol-3P, gluconate and adenine) were shared by all plant organs. A subset of 12 metabolites, including proline, were further evaluated as drought bioindicator candidates, with galactinol and gluconate emerging for vegetative parts while alanine seems informative of aerial part water status. In general, water limitation decreased the content of nitrogen compounds in aboveground tissues and increased the amounts of carbohydrates, especially in the sink organs. This study adds to our understanding of the metabolic responses of grasses to water limitation and identified potential bioindicators for drought in different plant organs.
Details
- Title
- Comparative Primary Metabolite Profiling of Setaria viridis Reveals Potential Markers to Water Limitation
- Authors/Creators
- Fernanda Alves de Freitas Guedes - Univ Fed Rio De Janeiro UFRJ, Inst Biol, Dept Genet, Av Prof Rodolpho Paulo Rocco,S-N CCS, BR-21941970 Rio De Janeiro, RJ, BrazilLuana Beatriz dos Santos Nascimento - Univ Fed Rio De Janeiro UFRJ, Programa Posgrad Biotecnol Vegetal, Av Carlos Chagas Filho,S-N CCS, BR-21941590 Rio De Janeiro, RJ, BrazilMara Priscila Costa - Univ Fed Rio De Janeiro UFRJ, Programa Posgrad Biotecnol Vegetal, Av Carlos Chagas Filho,S-N CCS, BR-21941590 Rio De Janeiro, RJ, BrazilAndrew Macrae - Univ Fed Rio De Janeiro UFRJ, Programa Posgrad Biotecnol Vegetal, Av Carlos Chagas Filho,S-N CCS, BR-21941590 Rio De Janeiro, RJ, BrazilMarcio Alves-Ferreira - Univ Fed Rio De Janeiro UFRJ, Inst Biol, Dept Genet, Av Prof Rodolpho Paulo Rocco,S-N CCS, BR-21941970 Rio De Janeiro, RJ, BrazilCamila Caldana - Natl Ctr Res Energy & Mat CNPEM, BR-13083100 Campinas, SP, BrazilFernanda Reinert - Univ Fed Rio De Janeiro UFRJ, Programa Posgrad Biotecnol Vegetal, Av Carlos Chagas Filho,S-N CCS, BR-21941590 Rio De Janeiro, RJ, Brazil
- Publication Details
- Agriculture (Basel), Vol.13(3), 660
- Publisher
- Mdpi
- Number of pages
- 19
- Identifiers
- 991005894545107891
- Copyright
- © 2023 by the authors.
- Murdoch Affiliation
- Food Futures Institute
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.49 Plant Stress Responses
- Web Of Science research areas
- Agronomy
- ESI research areas
- Agricultural Sciences