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Published (Version of Record)CC BY V4.0, Open Access
Journal article
Comparative analysis of malignant pleural effusion and peripheral blood reveals unique T cell signatures associated with survival in mesothelioma patients
Oxford open immunology, Vol.7(1), iqaf008
2025
Abstract
The success of cancer immunotherapies has highlighted the importance of monitoring the anti-tumour T cell response. Patients with mesothelioma frequently present with a malignant pleural effusion (MPE) that is commonly drained regularly to alleviate symptoms. As MPE contains tumour cells, T cells and cytokines, it provides a unique opportunity to sample immune events at the tumour site. However, there is minimal information on how MPE T cells are distinct from those in the blood, and whether T cell phenotypes unique to each compartment correlate with survival. We characterised T cell populations of matched MPE and blood from 31 mesothelioma patients using flow cytometry and bulk T cell receptor beta (TCRβ) sequencing. MPE CD8+ and CD4+ T cells displayed increased expression of PD-1, TIGIT, LAG-3 and TIM-3 compared to blood, with co-expression of inhibitory receptors greatest on MPE CD8+ T cells with a tissue resident memory T cell phenotype (CD69+CD103+). CD8+ TCRβ repertoires displayed clonal overlap between MPE and blood, suggesting that a majority of T cells traffic between these compartments. Finally, we show that high expression of PD-1 on circulating CD4+ T cells is an independent prognostic factor for poor survival in this patient group. This work suggests that MPE T cell phenotypes differ from those in circulation, with blood-based T cell subsets more sensitive predictors of outcome in this study.
Details
- Title
- Comparative analysis of malignant pleural effusion and peripheral blood reveals unique T cell signatures associated with survival in mesothelioma patients
- Authors/Creators
- Nicola Principe - Institute for Respiratory HealthKofi L P Stevens - Institute for Respiratory HealthAmber-Lee Phung - Institute for Respiratory HealthMelanie J Mccoy - The University of Western AustraliaJoel Kidman - Institute for Respiratory HealthAli IsmailAlistair M Cook - Institute for Respiratory HealthAbha Chopra - Murdoch UniversityMark Watson - Murdoch UniversityBruce W Robinson - Institute for Respiratory HealthJenette Creaney - Institute for Respiratory HealthYc Lee - Sir Charles Gairdner HospitalJason Waithman - The University of Western AustraliaW Joost Lesterhuis - The University of Western AustraliaRichard A Lake - Institute for Respiratory HealthAnna K Nowak - Institute for Respiratory HealthJonathan Chee - Institute for Respiratory HealthAlison M Mcdonnell - The University of Western Australia
- Publication Details
- Oxford open immunology, Vol.7(1), iqaf008
- Publisher
- Oxford University Press.
- Identifiers
- 991005850543807891
- Copyright
- © The Author(s) 2025.
- Murdoch Affiliation
- Personalised Medicine Centre
- Language
- English
- Resource Type
- Journal article
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