Journal article
Comparative analysis of the testis and ovary transcriptomes in Zebrafish by combining experimental and computational tools
Comparative and Functional Genomics, Vol.5(5), pp.403-418
2004
Abstract
Studies on the zebrafish model have contributed to our understanding of several important developmental processes, especially those that can be easily studied in the embryo. However, our knowledge on late events such as gonad differentiation in the zebrafish is still limited. Here we provide an analysis on the gene sets expressed in the adult zebrafish testis and ovary in an attempt to identify genes with potential role in (zebra)fish gonad development and function. We produced 10,533 expressed sequence tags (ESTs) from zebrafish testis or ovary and downloaded an additional 23,642 gonad-derived sequences from the zebrafish EST database. We clustered these sequences together with over 13,000 kidney-derived zebrafish ESTs to study partial transcriptomes for these three organs. We searched for genes with gonad-specific expression by screening macroarrays containing at least 2600 unique cDNA inserts with testis-, ovary- and kidney-derived cDNA probes. Clones hybridizing to only one of the two gonad probes were selected, and subsequently screened with computational tools to identify 72 genes with potentially testis-specific and 97 genes with potentially ovary-specific expression, respectively. PCR-amplification confirmed gonad-specificity for 21 of the 45 clones tested (all without known function). Our study, which involves over 47,000 EST sequences and specialized cDNA arrays, is the first analysis of adult organ transcriptomes of zebrafish at such a scale. The study of genes expressed in adult zebrafish testis and ovary will provide useful information on regulation of gene expression in teleost gonads and might also contribute to our understanding of the development and differentiation of reproductive organs in vertebrates.
Details
- Title
- Comparative analysis of the testis and ovary transcriptomes in Zebrafish by combining experimental and computational tools
- Authors/Creators
- Y. Li (Author/Creator) - Temasek Life Sciences LaboratoryJ.M. Chia (Author/Creator) - Genome Institute of SingaporeR. Bartfai (Author/Creator) - National University of SingaporeA. Christoffels (Author/Creator)G-H Yue (Author/Creator) - Temasek Life Sciences LaboratoryK. Ding (Author/Creator) - Temasek Life Sciences LaboratoryM.Y. Ho (Author/Creator) - Temasek Life Sciences LaboratoryJ.A. Hill (Author/Creator) - Temasek Life Sciences LaboratoryE. Stupka (Author/Creator) - Temasek Life Sciences LaboratoryL. Orbán (Author/Creator) - National University of Singapore
- Publication Details
- Comparative and Functional Genomics, Vol.5(5), pp.403-418
- Publisher
- Hindawi Publishing Corporation
- Identifiers
- 991005540032707891
- Copyright
- © 2004 Hindawi Publishing Corporation.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.2 Marine Biology
- 3.2.1123 Fish Reproductive Biology
- Web Of Science research areas
- Biochemistry & Molecular Biology
- Biotechnology & Applied Microbiology
- Genetics & Heredity
- ESI research areas
- Molecular Biology & Genetics