Comparative assessment of Nanotrap and polyethylene glycol-based virus concentration in wastewater samples

Kata Farkas; Jessica L Kevill; Rachel C Williams; Igor Pântea; Nicola Ridding; Kathryn Lambert-Slosarska; Nick Woodhall; Jasmine M S Grimsley; Matthew J Wade; Andrew C Singer; Andrew J Weightman; Gareth Cross; Davey L Jones

doi:10.1093/femsmc/xtae007

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Comparative assessment of Nanotrap and polyethylene glycol-based virus concentration in wastewater samples

Journal article

Open access

Peer reviewed

Comparative assessment of Nanotrap and polyethylene glycol-based virus concentration in wastewater samples

Kata Farkas, Jessica L Kevill, Rachel C Williams, Igor Pântea, Nicola Ridding, Kathryn Lambert-Slosarska, Nick Woodhall, Jasmine M S Grimsley, Matthew J Wade, Andrew C Singer, …

FEMS microbes, Vol.5, xtae007

2024

DOI: https://doi.org/10.1093/femsmc/xtae007

PMID: 38544682

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Abstract

sewage surveillance

enteric viruses

respiratory viruses

public health

concentration methods

Wastewater-based epidemiology is now widely used in many countries for the routine monitoring of SARS-CoV-2 and other viruses at a community level. However, efficient sample processing technologies are still under investigation. In this study, we compared the performance of the novel Nanotrap® Microbiome Particles (NMP) concentration method to the commonly used polyethylene glycol (PEG) precipitation method for concentrating viruses from wastewater and their subsequent quantification and sequencing. For this, we first spiked wastewater with SARS-CoV-2, influenza and measles viruses and norovirus and found that the NMP method recovered 0.4%-21% of them depending on virus type, providing consistent and reproducible results. Using the NMP and PEG methods, we monitored SARS-CoV-2, influenza A and B viruses, RSV, enteroviruses and norovirus GI and GII and crAssphage in wastewater using quantitative PCR (qPCR)-based methods and next-generation sequencing. Good viral recoveries were observed for highly abundant viruses using both methods; however, PEG precipitation was more successful in the recovery of low-abundance viruses present in wastewater. Furthermore, samples processed with PEG precipitation were more successfully sequenced for SARS-CoV-2 than those processed with the NMP method. Virus recoveries were enhanced by high sample volumes when PEG precipitation was applied. Overall, our results suggest that the NMP concentration method is a rapid and easy virus concentration method for viral targets that are abundant in wastewater, whereas PEG precipitation may be more suited to the recovery and analysis of low-abundance viruses and for next generation sequencing.

Details

Title: Comparative assessment of Nanotrap and polyethylene glycol-based virus concentration in wastewater samples
Authors/Creators: Kata Farkas - Bangor University
Jessica L Kevill - Bangor University
Rachel C Williams - Bangor University
Igor Pântea - Bangor University
Nicola Ridding - Bangor University
Kathryn Lambert-Slosarska - Bangor University
Nick Woodhall - Bangor University
Jasmine M S Grimsley - Health Data Research UK
Matthew J Wade - Newcastle University
Andrew C Singer - UK Centre for Ecology & Hydrology
Andrew J Weightman - Cardiff University
Gareth Cross - Welsh Government
Davey L Jones - Murdoch University, Centre for Sustainable Farming Systems
Publication Details: FEMS microbes, Vol.5, xtae007
Publisher: Oxford University Press on behalf of FEMS.
Identifiers: 991005652869807891
Murdoch Affiliation: Centre for Sustainable Farming Systems
Language: English
Resource Type: Journal article

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