Comparative genomic analysis of Asian haemorrhagic septicaemia-associated strains of Pasteurella multocida identifies more than 90 haemorrhagic septicaemia-specific genes

A.M. Moustafa; T. Seemann; S. Gladman; B. Adler; M. Harper; J.D. Boyce; M.D. Bennett

doi:10.1371/journal.pone.0130296

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Comparative genomic analysis of Asian haemorrhagic septicaemia-associated strains of Pasteurella multocida identifies more than 90 haemorrhagic septicaemia-specific genes

Journal article

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Comparative genomic analysis of Asian haemorrhagic septicaemia-associated strains of Pasteurella multocida identifies more than 90 haemorrhagic septicaemia-specific genes

A.M. Moustafa, T. Seemann, S. Gladman, B. Adler, M. Harper, J.D. Boyce and M.D. Bennett

PLOS ONE, Vol.10(7), e0130296

2015

DOI: https://doi.org/10.1371/journal.pone.0130296

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Abstract

Pasteurella multocida is the primary causative agent of a range of economically important diseases in animals, including haemorrhagic septicaemia (HS), a rapidly fatal disease of ungulates. There is limited information available on the diversity of P. multocida strains that cause HS. Therefore, we determined draft genome sequences of ten disease-causing isolates and two vaccine strains and compared these genomes using a range of bioinformatic analyses. The draft genomes of the 12 HS strains were between 2,298,035 and 2,410,300 bp in length. Comparison of these genomes with the North American HS strain, M1404, and other available P. multocida genomes (Pm70, 3480, 36950 and HN06) identified a core set of 1,824 genes. A set of 96 genes was present in all HS isolates and vaccine strains examined in this study, but absent from Pm70, 3480, 36950 and HN06. Moreover, 59 genes were shared only by the Asian B:2 strains. In two Pakistani isolates, genes with high similarity to genes in the integrative and conjugative element, ICEPmu1 from strain 36950 were identified along with a range of other antimicrobial resistance genes. Phylogenetic analysis indicated that the HS strains formed clades based on their country of isolation. Future analysis of the 96 genes unique to the HS isolates will aid the identification of HS-specific virulence attributes and facilitate the development of disease-specific diagnostic tests.

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Title: Comparative genomic analysis of Asian haemorrhagic septicaemia-associated strains of Pasteurella multocida identifies more than 90 haemorrhagic septicaemia-specific genes
Authors/Creators: A.M. Moustafa (Author/Creator) - Murdoch University
T. Seemann (Author/Creator) - Melbourne Bioinformatics
S. Gladman (Author/Creator) - Melbourne Bioinformatics
B. Adler (Author/Creator) - Australian Research Council
M. Harper (Author/Creator) - Australian Research Council
J.D. Boyce (Author/Creator) - Australian Research Council
M.D. Bennett (Author/Creator) - Murdoch University
Publication Details: PLOS ONE, Vol.10(7), e0130296
Publisher: Public Library of Science
Identifiers: 991005541212707891
Copyright: © 2015 Moustafa et al.
Murdoch Affiliation: School of Veterinary and Life Sciences
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.248 Sexually Transmitted Infections; 1.248.1656 Pasteurella Multocida
Web Of Science research areas: Microbiology
ESI research areas: Microbiology