Journal article
Comparative genomics and DNA array-based genotyping of pandemic Staphylococcus aureus strains encoding Panton-Valentine leukocidin
Clinical Microbiology and Infection, Vol.13(3), pp.236-249
2007
Abstract
Within the last few years, methicillin-resistant Staphylococcus aureus (MRSA) strains encoding Panton-Valentine leukocidin (PVL) have emerged and spread worldwide. This epidemic can be attributed to a small number of distinct clones. The present study used a novel assay, based on multiplex linear DNA amplification and subsequent microarray hybridisation, to simultaneously detect all relevant exotoxins, antimicrobial resistance determinants and the allelic variants of agr. The genes of the staphylococcal exotoxin-like (set) locus were also included for typing purposes. This assay, together with multilocus sequence typing (MLST) and spa typing, was applied to 56 clinical isolates and reference strains representing all major pandemic PVL-MRSA lineages, as well as to phylogenetically-related strains and putative ancestors. Array hybridisation results allowed the assignment of isolates to clonal groups, which were in accordance with MLST and spa typing data. Ten distinct clonal groups of PVL-MRSA (ST1, ST5, ST8, ST22, ST30, ST59/359, ST80/583, ST88, ST93 and ST152), including 12 MLST types, were identified and analysed with regard to resistance determinants and genes coding for exotoxins. The array hybridisation data confirmed that pandemic PVL-positive strains originate from very diverse genetic backgrounds, and provided insights into the evolution of some lineages. The DNA microarray technique provides a valuable epidemiological tool for the detailed characterisation of clinical isolates and comparison of strains at a global level.
Details
- Title
- Comparative genomics and DNA array-based genotyping of pandemic Staphylococcus aureus strains encoding Panton-Valentine leukocidin
- Authors/Creators
- S. Monecke (Author/Creator) - University Hospital Carl Gustav CarusB. Berger-Bächi (Author/Creator) - University of ZurichG. Coombs (Author/Creator) - Royal Perth HospitalA. Holmes (Author/Creator) - Health Protection AgencyI. Kay (Author/Creator) - Royal Perth HospitalA. Kearns (Author/Creator) - Health Protection AgencyH-J Linde (Author/Creator) - University of RegensburgF. O'Brien (Author/Creator) - Curtin UniversityP. Slickers (Author/Creator) - CLONDIAG chip technologies GmbH, Jena, GermanyR. Ehricht (Author/Creator) - CLONDIAG chip technologies GmbH, Jena, Germany
- Publication Details
- Clinical Microbiology and Infection, Vol.13(3), pp.236-249
- Publisher
- Elsevier
- Identifiers
- 991005543616707891
- Copyright
- © 2007 European Society of Clinical Microbiology and Infectious Diseases.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.23 Antibiotics & Antimicrobials
- 1.23.173 MRSA and VRE
- Web Of Science research areas
- Infectious Diseases
- Microbiology
- ESI research areas
- Immunology