Files and links (1)
CC BY-NC-ND V4.0, Open Access
Journal article
Comparative genomics of Staphylococcus aureus strains from wild birds and pig farms elucidates levels of mobilomes, antibiotic pressure and host adaptation
Journal of global antimicrobial resistance, Vol.36, pp.142-150
2024
PMID: 38128728
Abstract
This study characterized the resistome, mobilome and phylogenomic relatedness of Staphylococcus aureus strains previously obtained from healthy nestling storks (HNS), pigs (HP) and pig farmers (HPF) to analyse possible transmission pathways of S. aureus with implications for the spread of antimicrobial resistance.
The genomic contents of 52 S. aureus strains obtained from the nasal cavity of HNS, HP and HPF in Spain were sequenced using the Illumina NextSeq platform to characterize their resistome, virulome and mobile genetic elements. The relatedness of strains was assessed by core-genome single nucleotide polymorphisms (SNPs).
The frequencies of multidrug-resistance phenotype and transposons were significantly lower in strains from HNS than in those from HP and HPF (P < 0.005). However, the presence of human immune evasion cluster genes in S. aureus strains from HNS was significantly higher than in those from HP and HPF (P < 0.005). Interestingly, the frequencies of plasmids and phages were not significantly associated with the host (P > 0.05). The phylogenetic analysis identified a cluster of all the MSSA-CC398 strains carrying φSa3 and ermT on rep13 separately from the two MRSA-CC398 strains (carrying ermT on repUS18). Highly related MRSA-CC398 strains were detected in some pigs and related farmers (<10 SNPs).
This study confirms high-level antibiotic selection in S. aureus in HP and HPF in comparison to HNS. Furthermore, our findings highlight the continuous transmission of MRSA-CC398 in the pig-to-human interface and MSSA-CC398 with human adaptation markers in HNS. Molecular surveillance of S. aureus using the One Health model is required to establish appropriate control strategies.
Details
- Title
- Comparative genomics of Staphylococcus aureus strains from wild birds and pig farms elucidates levels of mobilomes, antibiotic pressure and host adaptation
- Authors/Creators
- Idris Nasir Abdullahi - Universidad de La RiojaCarmen Lozano - Universidad de La RiojaMyriam Zarazaga - Universidad de La RiojaCarmen Simón - Universidad de ZaragozaUrsula Höfle - IRECRaphael N Sieber - Department of Bacteria, Parasites and Fungi, Statens Serum Institut, Copenhagen, DenmarkJavier Latorre-Fernández - Universidad de La RiojaMarc Stegger - Murdoch UniversityCarmen Torres - Universidad de La Rioja
- Publication Details
- Journal of global antimicrobial resistance, Vol.36, pp.142-150
- Publisher
- Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy.
- Identifiers
- 991005634766907891
- Copyright
- © 2023 The Authors.
- Murdoch Affiliation
- Harry Butler Institute
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Metrics
41 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.23 Antibiotics & Antimicrobials
- 1.23.173 MRSA and VRE
- Web Of Science research areas
- Infectious Diseases
- Pharmacology & Pharmacy
- ESI research areas
- Immunology