Journal article
Comparative proteomic analysis of grain development in two spring wheat varieties under drought stress
Analytical and Bioanalytical Chemistry, Vol.402(3), pp.1297-1313
2012
Abstract
Two spring wheat varieties Ningchun 4 and Chinese Spring with good and poor resistance to abiotic stress, respectively, were used to investigate proteomic changes in the developing grains under drought stress by a comparative proteomics approach. A total of 152 protein spots showed at least twofold differences in abundance on two-dimensional electrophoresis (2-DE) maps, of which 28 and 68 protein spots were identified by MALDI-TOF and MALDI-TOF/TOF mass spectrometry, respectively. Of the 96 identified protein spots, six different expression patterns were found and they were involved in stress/defense/detoxification, carbohydrate metabolism, photosynthesis, nitrogen metabolism, storage proteins and some other important functions. Comparative proteomic analysis revealed that under the drought conditions the decreased degree of ascorbate peroxidases was more significant in Chinese Spring than in Ningchun 4 during grain development whereas translationally controlled tumor protein, which was significantly upregulated at 14 DAF, was present in Ningchun 4 and absent in Chinese Spring. The Rubisco large subunit displayed an upregulated expression pattern in Ningchun 4. In addition, two drought-tolerant proteins, triosephosphate isomerase and oxygen-evolving complex showed B and F type expression patterns in Chinese Spring, but D and B types in Ningchun 4, respectively. These differentially expressed proteins might be responsible for the stronger drought resistance of Ningchun 4 compared to Chinese Spring.
Details
- Title
- Comparative proteomic analysis of grain development in two spring wheat varieties under drought stress
- Authors/Creators
- P. Ge (Author/Creator)C. Ma (Author/Creator)S. Wang (Author/Creator)L. Gao (Author/Creator)X. Li (Author/Creator)G. Guo (Author/Creator)W. Ma (Author/Creator)Y. Yan (Author/Creator)
- Publication Details
- Analytical and Bioanalytical Chemistry, Vol.402(3), pp.1297-1313
- Publisher
- Springer-Verlag
- Identifiers
- 991005543381107891
- Copyright
- © Springer-Verlag 2011
- Murdoch Affiliation
- Centre for Comparative Genomics
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.49 Plant Stress Responses
- Web Of Science research areas
- Biochemical Research Methods
- Chemistry, Analytical
- ESI research areas
- Chemistry