Journal article
Comparative proteomic analysis of salt response proteins in seedling roots of two wheat varieties
Journal of Proteomics, Vol.75(6), pp.1867-1885
2012
Abstract
A comparative proteomic analysis was made of salt response in seedling roots of wheat cultivars Jing-411 (salt tolerant) and Chinese Spring (salt sensitive) subjected to a range of salt stress concentrations (0.5%, 1.5% and 2.5%) for 2. days. One hundred and ninety eight differentially expressed protein spots (DEPs) were located with at least two-fold differences in abundance on 2-DE maps, of which 144 were identified by MALDI-TOF-TOF MS. These proteins were involved primarily in carbon metabolism (31.9%), detoxification and defense (12.5%), chaperones (5.6%) and signal transduction (4.9%). Comparative analysis showed that 41 DEPs were salt responsive with significant expression changes in both varieties under salt stress, and 99 (52 in Jing-411 and 47 in Chinese Spring) were variety specific. Only 15 and 9 DEPs in Jing-411 and Chinese Spring, respectively, were up-regulated in abundance under all three salt concentrations. All dynamics of the DEPs were analyzed across all treatments. Some salt responsive DEPs, such as guanine nucleotide-binding protein subunit beta-like protein, RuBisCO large subunit-binding protein subunit alpha and pathogenesis related protein 10, were up-regulated significantly in Jing-411 under all salt concentrations, whereas they were down-regulated in salinity-stressed Chinese Spring.
Details
- Title
- Comparative proteomic analysis of salt response proteins in seedling roots of two wheat varieties
- Authors/Creators
- G. Guo (Author/Creator)P. Ge (Author/Creator)C. Ma (Author/Creator)X. Li (Author/Creator)D. Lv (Author/Creator)S. Wang (Author/Creator)W. Ma (Author/Creator)Y. Yan (Author/Creator)
- Publication Details
- Journal of Proteomics, Vol.75(6), pp.1867-1885
- Publisher
- Elsevier BV
- Identifiers
- 991005543391207891
- Copyright
- © 2012 Elsevier B.V.
- Murdoch Affiliation
- State Agricultural Biotechnology Centre
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.49 Plant Stress Responses
- Web Of Science research areas
- Biochemical Research Methods
- ESI research areas
- Biology & Biochemistry