Journal article
Comparative spatial and temporal localisation of perlecan, aggrecan and type I, II and IV collagen in the ovine meniscus: an ageing study
Histochemistry and Cell Biology, Vol.124(3-4), pp.225-235
2005
Abstract
This is the first study to immunolocalise perlecan in meniscal tissues and to demonstrate how its localisation varied with ageing relative to aggrecan and type I, II and IV collagen. Perlecan was present in the middle and inner meniscal zones where it was expressed by cells of an oval or rounded morphology. Unlike the other components visualised in this study, perlecan was strongly cell associated and its levels fell significantly with age onset and cell number decline. The peripheral outer meniscal zones displayed very little perlecan staining other than in small blood vessels. Picrosirius red staining viewed under polarised light strongly delineated complex arrangements of slender discrete randomly oriented collagen fibre bundles as well as transverse, thick, strongly oriented, collagen tie bundles in the middle and outer meniscal zones. The collagen fibres demarcated areas of the meniscus which were rich in anionic toluidine blue positive proteoglycans; immunolocalisations confirmed the presence of aggrecan and perlecan. When meniscal sections were examined macroscopically, type II collagen localisation in the inner meniscal zone was readily evident in the 2- to 7-day-old specimens; this became more disperse in the older meniscal specimens. Type I collagen had a widespread distribution in all meniscal zones at all time points. Type IV collagen was strongly associated with blood vessels in the 2- to 7-day-old meniscal specimens but was virtually undetectable at the later time points (>7 month).
Details
- Title
- Comparative spatial and temporal localisation of perlecan, aggrecan and type I, II and IV collagen in the ovine meniscus: an ageing study
- Authors/Creators
- J. Melrose (Author/Creator) - University Clinic of TraumatologyS. Smith (Author/Creator) - University Clinic of TraumatologyM. Cake (Author/Creator) - Murdoch UniversityR. Read (Author/Creator) - Murdoch UniversityJ. Whitelock (Author/Creator) - UNSW Sydney
- Publication Details
- Histochemistry and Cell Biology, Vol.124(3-4), pp.225-235
- Publisher
- Springer Verlag
- Identifiers
- 991005543240907891
- Copyright
- © Springer-Verlag 2005
- Murdoch Affiliation
- School of Veterinary and Biomedical Sciences
- Language
- English
- Resource Type
- Journal article
Metrics
31 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.34 Orthopedics
- 1.34.440 Anterior Cruciate Ligament
- Web Of Science research areas
- Cell Biology
- Microscopy
- ESI research areas
- Biology & Biochemistry