Journal article
Comparative spatial lipidomics analysis reveals cellular lipid remodelling in different developmental zones of barley roots in response to salinity
Plant, Cell & Environment, Vol.43(2), pp.327-343
2020
Abstract
Salinity‐induced metabolic, ionic, and transcript modifications in plants have routinely been studied using whole plant tissues, which do not provide information on spatial tissue responses. The aim of this study was to assess the changes in the lipid profiles in a spatial manner and to quantify the changes in the elemental composition in roots of seedlings of four barley cultivars before and after a short‐term salt stress. We used a combination of liquid chromatography–tandem mass spectrometry, inductively coupled plasma mass spectrometry, matrix‐assisted laser desorption/ionization mass spectrometry imaging, and reverse transcription – quantitative real time polymerase chain reaction platforms to examine the molecular signatures of lipids, ions, and transcripts in three anatomically different seminal root tissues before and after salt stress. We found significant changes to the levels of major lipid classes including a decrease in the levels of lysoglycerophospholipids, ceramides, and hexosylceramides and an increase in the levels of glycerophospholipids, hydroxylated ceramides, and hexosylceramides. Our results revealed that modifications to lipid and transcript profiles in plant roots in response to a short‐term salt stress may involve recycling of major lipid species, such as phosphatidylcholine, via resynthesis from glycerophosphocholine.
Details
- Title
- Comparative spatial lipidomics analysis reveals cellular lipid remodelling in different developmental zones of barley roots in response to salinity
- Authors/Creators
- L.D. Sarabia (Author/Creator)B.A. Boughton (Author/Creator)T. Rupasinghe (Author/Creator)D.L. Callahan (Author/Creator)C.B. Hill (Author/Creator)U. Roessner (Author/Creator)
- Publication Details
- Plant, Cell & Environment, Vol.43(2), pp.327-343
- Publisher
- Blackwell Publishing Inc.
- Identifiers
- 991005544232807891
- Copyright
- © 2019 John Wiley & Sons Ltd
- Murdoch Affiliation
- School of Veterinary and Life Sciences
- Language
- English
- Resource Type
- Journal article
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Source: InCites
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- Collaboration types
- Domestic collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.49 Plant Stress Responses
- Web Of Science research areas
- Plant Sciences
- ESI research areas
- Plant & Animal Science