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Comparative studies on the axenic in vitro cultivation of Giardia of human and canine origin: evidence for intraspecific variation
Journal article   Peer reviewed

Comparative studies on the axenic in vitro cultivation of Giardia of human and canine origin: evidence for intraspecific variation

B.P. Meloni and R.C.A. Thompson
Transactions of the Royal Society of Tropical Medicine and Hygiene, Vol.81(4), pp.637-640
1987
DOI: https://doi.org/10.1016/0035-9203(87)90438-X
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Abstract

Comparative studies were carried out on the in vitro cultivation of Giardia duodenalis from dogs and humans. Cultures were initiated with trophozoites obtained by artificial excystation of cysts present in human or canine faecal specimens, or using trophozoites collected from the small intestine of dogs postmortem. 12 new human isolates of G. duodenalis were established in axenic culture from cysts present in faecal specimens, and successfully cryopreserved, an overall success rate for in vitro establishment of Giardia from cysts of approximately 44%. In contrast, not one of 24 canine isolates, whether of faecal or intestinal origin, became established in vitro. Since identical media and culture conditions were used for the cultivation of both human and canine isolates, the results may reflect strain differences. The zoonotic significance of such intraspecific variation is discussed.

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Source: InCites

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Citation topics
1 Clinical & Life Sciences
1.246 Diarrheal Diseases
1.246.985 Cryptosporidium
Web Of Science research areas
Public, Environmental & Occupational Health
Tropical Medicine
ESI research areas
Social Sciences, general
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