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Comparative transcriptome analysis identified candidate genes for late leaf spot resistance and cause of defoliation in groundnut.pdf
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Journal article
Comparative transcriptome analysis identified candidate genes for late leaf spot resistance and cause of defoliation in groundnut
International Journal of Molecular Sciences, Vol.22(9), Article 4491
2021
Abstract
Late leaf spot (LLS) caused by fungus Nothopassalora personata in groundnut is responsible for up to 50% yield loss. To dissect the complex nature of LLS resistance, comparative transcriptome analysis was performed using resistant (GPBD 4), susceptible (TAG 24) and a resistant introgression line (ICGV 13208) and identified a total of 12,164 and 9954 DEGs (differentially expressed genes) respectively in A- and B-subgenomes of tetraploid groundnut. There were 135 and 136 unique pathways triggered in A- and B-subgenomes, respectively, upon N. personata infection. Highly upregulated putative disease resistance genes, an RPP-13 like (Aradu.P20JR) and a NBS-LRR (Aradu.Z87JB) were identified on chromosome A02 and A03, respectively, for LLS resistance. Mildew resistance Locus (MLOs)-like proteins, heavy metal transport proteins, and ubiquitin protein ligase showed trend of upregulation in susceptible genotypes, while tetratricopeptide repeats (TPR), pentatricopeptide repeat (PPR), chitinases, glutathione S-transferases, purple acid phosphatases showed upregulation in resistant genotypes. However, the highly expressed ethylene responsive factor (ERF) and ethylene responsive nuclear protein (ERF2), and early responsive dehydration gene (ERD) might be related to the possible causes of defoliation in susceptible genotypes. The identified disease resistance genes can be deployed in genomics-assisted breeding for development of LLS resistant cultivars to reduce the yield loss in groundnut.
Details
- Title
- Comparative transcriptome analysis identified candidate genes for late leaf spot resistance and cause of defoliation in groundnut
- Authors/Creators
- S.S. Gangurde (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsS.N. Nayak (Author/Creator) - University of Agricultural Sciences, DharwadP. Joshi (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsS. Purohit (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsH.K. Sudini (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsA. Chitikineni (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsY. Hong (Author/Creator) - Guangdong Academy of Agricultural SciencesB. Guo (Author/Creator) - Agricultural Research ServiceX. Chen (Author/Creator) - Guangdong Academy of Agricultural SciencesM.K. Pandey (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsR.K. Varshney (Author/Creator) - International Crops Research Institute for the Semi-Arid Tropics
- Publication Details
- International Journal of Molecular Sciences, Vol.22(9), Article 4491
- Publisher
- MDPI
- Identifiers
- 991005543976307891
- Copyright
- © 2021 by the authors
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.96 QTL
- Web Of Science research areas
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- Chemistry, Multidisciplinary
- ESI research areas
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