Journal article
Comparative transcriptome analysis of aerial and subterranean pods development provides insights into seed abortion in peanut
Plant Molecular Biology, Vol.85(4-5), pp.395-409
2014
Abstract
The peanut is a special plant for its aerial flowering but subterranean fructification. The failure of peg penetration into the soil leads to form aerial pod and finally seed abortion. However, the mechanism of seed abortion during aerial pod development remains obscure. Here, a comparative transcriptome analysis between aerial and subterranean pods at different developmental stages was produced using a customized NimbleGen microarray representing 36,158 unigenes. By comparing 4 consecutive time-points, totally 6,203 differentially expressed genes, 4,732 stage-specific expressed genes and 2,401 specific expressed genes only in aerial or subterranean pods were identified in this study. Functional annotation showed their mainly involvement in biosynthesis, metabolism, transcription regulation, transporting, stress response, photosynthesis, signal transduction, cell division, apoptosis, embryonic development, hormone response and light signaling, etc. Emphasis was focused on hormone response, cell apoptosis, embryonic development and light signaling relative genes. These genes might function as potential candidates to provide insights into seed abortion during aerial pod development. Ten candidate genes were validated by Real-time RT-PCR. Additionally, consistent with up-regulation of auxin response relative genes in aerial pods, endogenous IAA content was also significantly increased by HPLC analysis. This study will further provide new molecular insight that auxin and auxin response genes potentially contribute to peanut seed and pod development.
Details
- Title
- Comparative transcriptome analysis of aerial and subterranean pods development provides insights into seed abortion in peanut
- Authors/Creators
- W. Zhu (Author/Creator) - Guangdong Academy of Agricultural SciencesX. Chen (Author/Creator) - Guangdong Academy of Agricultural SciencesH. Li (Author/Creator) - Guangdong Academy of Agricultural SciencesF. Zhu (Author/Creator) - Guangdong Academy of Agricultural SciencesY. Hong (Author/Creator) - Guangdong Academy of Agricultural SciencesR.K. Varshney (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsX. Liang (Author/Creator) - Guangdong Academy of Agricultural Sciences
- Publication Details
- Plant Molecular Biology, Vol.85(4-5), pp.395-409
- Publisher
- Springer Link
- Identifiers
- 991005541083607891
- Copyright
- © 2014 The Author(s).
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.159 Arabidopsis
- Web Of Science research areas
- Biochemistry & Molecular Biology
- Plant Sciences
- ESI research areas
- Plant & Animal Science