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Comparison of alcohol shock enrichment and selective enrichment for the isolation of Clostridium difficile
Journal article   Peer reviewed

Comparison of alcohol shock enrichment and selective enrichment for the isolation of Clostridium difficile

T.V. Riley, J.S. Brazier, H. Hassan, K. Williams and K.D. Phillips
Epidemiology and Infection, Vol.99(02), pp.355-359
1987
DOI: https://doi.org/10.1017/S0950268800067832
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Abstract

Two enrichment methods were compared for their ability to recover Clostridium difficile from stool samples. One method used selective enrichment in an antibiotic-containing broth followed by detection with a latex particle agglutination (LPA) reagent. The other used enrichment in a non-selective broth following treatment of the specimen with alcohol. With clinical specimens enrichment culture was significantly more successful at detecting C. difficile than direct plating. Alcohol shock enrichment was twice as effective as direct culture, while selective broth enrichment was three times more effective. The use of LPA for screening selective enrichment broths for C. difficile should prove a cost-effective measure as only positive broths (about 20%) require subculture for confirmation.

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Source: InCites

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24 Record Views
23 Times Cited - Web of Science

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Citation topics
1 Clinical & Life Sciences
1.120 Inflammatory Bowel Diseases & Infections
1.120.1133 Clostridium Infections
Web Of Science research areas
Infectious Diseases
Public, Environmental & Occupational Health
ESI research areas
Social Sciences, general
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