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CC BY V4.0, Open Access
Journal article
Comparison of in vitro growth characteristics of Cryptosporidium hominis (IdA15G1) and Cryptosporidium parvum (Iowa-IIaA17G2R1 and IIaA18G3R1)
Parasitology research (1987), Vol.122(12), pp.2891-2905
2023
PMID: 37776335
Abstract
Cryptosporidium is a major cause of diarrhoeal disease and mortality in young children in resource-poor countries, for which no vaccines or adequate therapeutic options are available. Infection in humans is primarily caused by two species: C. hominis and C. parvum . Despite C. hominis being the dominant species infecting humans in most countries, very little is known about its growth characteristics and life cycle in vitro, given that the majority of our knowledge of the in vitro development of Cryptosporidium has been based on C. parvum . In the present study, the growth and development of two C. parvum isolates (subtypes Iowa-IIaA17G2R1 and IIaA18G3R1) and one C. hominis isolate (subtype IdA15G1) in HCT-8 cells were examined and compared at 24 h and 48 h using morphological data acquired with scanning electron microscopy. Our data indicated no significant differences in the proportion of meronts or merozoites between species or subtypes at either time-point. Sexual development was observed at the 48-h time-point across both species through observations of both microgamonts and macrogamonts, with a higher frequency of macrogamont observations in C. hominis (IdA15G1) cultures at 48-h post-infection compared to both C. parvum subtypes. This corresponded to differences in the proportion of trophozoites observed at the same time point. No differences in proportion of microgamonts were observed between the three subtypes, which were rarely observed across all cultures. In summary, our data indicate that asexual development of C. hominis is similar to that of C. parvum, while sexual development is accelerated in C. hominis. This study provides new insights into differences in the in vitro growth characteristics of C. hominis when compared to C. parvum , which will facilitate our understanding of the sexual development of both species.
Details
- Title
- Comparison of in vitro growth characteristics of Cryptosporidium hominis (IdA15G1) and Cryptosporidium parvum (Iowa-IIaA17G2R1 and IIaA18G3R1)
- Authors/Creators
- Samantha Gunasekera - Murdoch UniversityPeta L. Clode - The University of Western AustraliaBrendon King - South Australian Water CorporationPaul Monis - South Australian Water CorporationBenjamin Thierry - University of South AustraliaJillian M. Carr - Flinders UniversityAbha Chopra - Murdoch University, Centre for Molecular Medicine and Innovative TherapeuticsMark Watson - Murdoch University, Centre for Molecular Medicine and Innovative TherapeuticsMark O’Dea - Murdoch UniversityNawal Hijjawi - Hashemite UniversityUna Ryan - Murdoch University, Centre for Biosecurity and One Health
- Publication Details
- Parasitology research (1987), Vol.122(12), pp.2891-2905
- Publisher
- Springer
- Number of pages
- 15
- Grant note
- Murdoch Strategic Scholarship University, State and Commonwealth Governments We thank Dr Andrew Ball from Water New South Wales, Dr Nicholas Crosbie from Melbourne Water, Dr Paul Fisher from Seqwater, and Dr Leon van der Linden from South Australian Water Corporation for their role as industry partners and contribution to funding.
- Identifiers
- 991005609164107891
- Copyright
- © 2023 The Author(s)
- Murdoch Affiliation
- College of Environmental and Life Sciences
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
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- Citation topics
- 1 Clinical & Life Sciences
- 1.246 Diarrheal Diseases
- 1.246.985 Cryptosporidium
- Web Of Science research areas
- Parasitology
- ESI research areas
- Microbiology