Comparison of low molecular weight glutenin subunits identified by SDS-PAGE, 2-DE, MALDI-TOF-MS and PCR in common wheat

L. Liu; T.M. Ikeda; G. Branlard; R.J. Peña; W.J. Rogers; S.E. Lerner; M.A. Kolman; X. Xia; L. Wang; W. Ma; R. Appels; H. Yoshida; A. Wang; Y. Yan; Z. He

doi:10.1186/1471-2229-10-124

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Comparison of low molecular weight glutenin subunits identified by SDS-PAGE, 2-DE, MALDI-TOF-MS and PCR in common wheat

Journal article

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Comparison of low molecular weight glutenin subunits identified by SDS-PAGE, 2-DE, MALDI-TOF-MS and PCR in common wheat

L. Liu, T.M. Ikeda, G. Branlard, R.J. Peña, W.J. Rogers, S.E. Lerner, M.A. Kolman, X. Xia, L. Wang, W. Ma, …

BMC Plant Biology, Vol.10, Article number: 124

2010

DOI: https://doi.org/10.1186/1471-2229-10-124

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Abstract

Low-molecular-weight glutenin subunits (LMW-GS) play a crucial role in determining end-use quality of common wheat by influencing the viscoelastic properties of dough. Four different methods - sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), two-dimensional gel electrophoresis (2-DE, IEF × SDS-PAGE), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and polymerase chain reaction (PCR), were used to characterize the LMW-GS composition in 103 cultivars from 12 countries.

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Title: Comparison of low molecular weight glutenin subunits identified by SDS-PAGE, 2-DE, MALDI-TOF-MS and PCR in common wheat
Authors/Creators: L. Liu (Author/Creator) - Civil Aviation Authority
T.M. Ikeda (Author/Creator) - National Agriculture and Food Research Organization
G. Branlard (Author/Creator) - Institut National de la Recherche Agronomique
R.J. Peña (Author/Creator) - Centro Internacional de Mejoramiento de Maíz Y Trigo
W.J. Rogers (Author/Creator) - Universidad Nacional del Centro de la Provincia de Buenos Aires
S.E. Lerner (Author/Creator) - Universidad Nacional del Centro de la Provincia de Buenos Aires
M.A. Kolman (Author/Creator)
X. Xia (Author/Creator) - Civil Aviation Authority
L. Wang (Author/Creator) - Civil Aviation Authority
W. Ma (Author/Creator) - Western Australia Department of Agriculture and Food, State Agriculture Biotechnology Center, Murdoch University, Murdoch, Australia
R. Appels (Author/Creator) - Western Australia Department of Agriculture and Food, State Agriculture Biotechnology Center, Murdoch University, Murdoch, Australia
H. Yoshida (Author/Creator) - National Agriculture and Food Research Organization
A. Wang (Author/Creator) - Capital Normal University
Y. Yan (Author/Creator) - Capital Normal University
Z. He (Author/Creator) - Centro Internacional de Mejoramiento de Maíz Y Trigo
Publication Details: BMC Plant Biology, Vol.10, Article number: 124
Publisher: BioMed Central
Identifiers: 991005541397707891
Copyright: © 2010 Liu et al
Murdoch Affiliation: State Agricultural Biotechnology Centre
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.85 Food Science & Technology; 3.85.99 Cereal Starch Properties
Web Of Science research areas: Plant Sciences
ESI research areas: Plant & Animal Science