Journal article
Comparison of manual and laboratory PCV and total protein using EDTA and lithium heparin canine samples
Journal of Small Animal Practice, Vol.55(5), pp.258-264
2014
Abstract
Objectives: To evaluate if lithium heparin (LiH) and potassium ethylenediaminetetraacetic acid (EDTA) can be used interchangeably to obtain packed cell volume (PCV) and total protein by refractometry (TPr), and to compare those values with laboratory-derived haematocrit (Hct) and total protein (TP) concentration, respectively, in canine blood samples. Methods: Blood samples taken in LiH and EDTA were manually assessed for PCV and TPr. Results were correlated to Hct and TP. Results: 238 EDTA and corresponding serum/LiH samples were obtained. There was excellent correlation but statistically significant difference between LiH and EDTA PCV (n=43). LiH and EDTA TPr (n=43) were excellently correlated without significant difference. PCV and Hct (n=176) were excellently correlated without significant difference. LiH (n=105) and serum (n=133) TP was respectively fairly or well correlated with TPr but with significant differences. An increase in cholesterol of 1 mmol/L was associated with a mean independent increase in TPr of approximately 1g/L. Clinical Significance: LiH and EDTA can be used interchangeably for TPr. Although TPr and serum/plasma TP were correlated, there were statistically significant differences that could impact on clinical decision making. TPr is increased by cholesterol but this alone could not account for the magnitude of the difference observed.
Details
- Title
- Comparison of manual and laboratory PCV and total protein using EDTA and lithium heparin canine samples
- Authors/Creators
- A. Tamborini (Author/Creator) - University College DublinS. Papakonstantinou (Author/Creator) - University College DublinA Brown (Author/Creator) - Murdoch UniversityS. Brennan (Author/Creator) - University College DublinR.E. Shiel (Author/Creator) - Murdoch UniversityJ.N. Mills (Author/Creator) - Murdoch UniversityC.T. Mooney (Author/Creator) - University College Dublin
- Publication Details
- Journal of Small Animal Practice, Vol.55(5), pp.258-264
- Publisher
- Blackwell Publishing
- Identifiers
- 991005545231807891
- Copyright
- © 2014 British Small Animal Veterinary Association.
- Murdoch Affiliation
- School of Veterinary and Life Sciences
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
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- Citation topics
- 1 Clinical & Life Sciences
- 1.307 Laboratory Medicine
- 1.307.1039 Lab Quality Control
- Web Of Science research areas
- Veterinary Sciences
- ESI research areas
- Plant & Animal Science