Comparison of molecular and McMaster microscopy techniques to confirm the presence of naturally acquired strongylid nematode infections in sheep

J.P.A. Sweeny; I.D. Robertson; U.M. Ryan; C. Jacobson; R.G. Woodgate

doi:10.1016/j.molbiopara.2011.07.007

Back

Comparison of molecular and McMaster microscopy techniques to confirm the presence of naturally acquired strongylid nematode infections in sheep

Journal article

Open access

Peer reviewed

Comparison of molecular and McMaster microscopy techniques to confirm the presence of naturally acquired strongylid nematode infections in sheep

J.P.A. Sweeny, I.D. Robertson, U.M. Ryan, C. Jacobson and R.G. Woodgate

Molecular and Biochemical Parasitology, Vol.180(1), pp.62-67

2011

DOI: https://doi.org/10.1016/j.molbiopara.2011.07.007

Files and links (2)

pdf

microscopy_techniques.pdfDownload View

Author’s Version Open Access

url

Link to Published Version *Subscription may be requiredView

Abstract

Patent strongylid nematode infections were identified using McMaster worm egg counts (WEC) and PCR assays (ITS-2 nuclear ribosomal DNA) to screen genomic DNA extracted directly from lamb faecal samples. Lambs from four different farms in southern Western Australia were sampled rectally on two separate occasions, with McMaster WECs and PCRs conducted on a total of 858 samples. Negative controls (n = 96) (WEC < 50 eggs per gram [epg]) and positive controls (n = 96) (faecal samples spiked with a 100 μL suspension of third-stage larvae (L3) containing approximately equal proportions of Teladorsagia circumcincta, Trichostrongylus colubriformis, Haemonchus contortus, Oesophagostomum spp. and Chabertia ovina) were generated. All control samples amplified in accordance with positive controls. High levels of agreement (Kappa values ≥ 0.93) were identified between the two diagnostic tests. PCRs detected an additional 2.0% of samples as strongylid-positive but there was no significant difference in the number of strongylid-positive samples identified using PCR or McMaster WEC.

Details

Title: Comparison of molecular and McMaster microscopy techniques to confirm the presence of naturally acquired strongylid nematode infections in sheep
Authors/Creators: J.P.A. Sweeny (Author/Creator) - Murdoch University
I.D. Robertson (Author/Creator) - Murdoch University
U.M. Ryan (Author/Creator) - Murdoch University
C. Jacobson (Author/Creator) - Murdoch University
R.G. Woodgate (Author/Creator) - Agriculture and Food
Publication Details: Molecular and Biochemical Parasitology, Vol.180(1), pp.62-67
Publisher: Elsevier B.V.
Identifiers: 991005543933307891
Copyright: 2011 Elsevier B.V.
Murdoch Affiliation: School of Veterinary and Biomedical Sciences
Language: English
Resource Type: Journal article

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites

Metrics

498 File views/ downloads

100 Record Views

27 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.163 Parasitology - General; 1.163.1022 Anthelmintic Resistance
Web Of Science research areas: Biochemistry & Molecular Biology; Parasitology
ESI research areas: Microbiology