Journal article
Comparison of three different PCR protocols for the detection of ferlaviruses
BMC Veterinary Research, Vol.15(1), Article number: 281
2019
Abstract
Background
Ferlaviruses are important pathogens in snakes often associated with respiratory and neurological disease. The detection of ferlaviral RNA by PCR is considered to be the most reliable method for the diagnosis of infection. The PCRs that have been used most commonly for this purpose have not been properly assessed to determine their sensitivity, specificity and ability to detect the known genetic diversity of this group of viruses. The aim of this study was to compare three published PCR protocols so that a single method could be recommended to laboratories that perform this testing.
Results
Comparisons were carried out using cell culture isolates and tissues from snakes infected with specific virus genotypes. A single round PCR targeting a short segment of the viral polymerase (L) gene provided the highest sensitivity and specificity, and detected isolated ferlaviruses from all four described genogroups, as well as from tissues of infected snakes.
Conclusion
A broadly-reactive PCR for the detection of all known ferlaviruses was found to provide a good combination of detection limit, specificity and speed. Based on these criteria, this method is recommended for the diagnosis of ferlavirus infections.
Details
- Title
- Comparison of three different PCR protocols for the detection of ferlaviruses
- Authors/Creators
- E. Kolesnik (Author/Creator) - Laboklin (Germany)T.H. Hyndman (Author/Creator) - Murdoch UniversityE. Müller (Author/Creator) - Laboklin (Germany)M. Pees (Author/Creator) - Leipzig UniversityR.E. Marschang (Author/Creator) - Laboklin (Germany)
- Publication Details
- BMC Veterinary Research, Vol.15(1), Article number: 281
- Publisher
- BioMed Central
- Identifiers
- 991005540210607891
- Copyright
- © The Author(s). 2019
- Murdoch Affiliation
- School of Veterinary Medicine
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
56 File views/ downloads
75 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Industry collaboration
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.35 Zoology & Animal Ecology
- 3.35.683 Reptile Ecology
- Web Of Science research areas
- Veterinary Sciences
- ESI research areas
- Plant & Animal Science