Journal article
Comparison of two culture techniques used to detect environmental contamination with Salmonella enterica in a large-animal hospital
Journal of the South African Veterinary Association, Vol.86(1), a1292
2015
Abstract
Salmonellosis is a common healthcare-associated infection in large-animal hospitals, and surveillance for Salmonella is an integral part of comprehensive infection control programmes in populations at risk. The present study compares the effectiveness of two culture techniques for recovery of Salmonella from environmental samples obtained in a large-animal referral veterinary hospital during a Salmonella outbreak. Environmental samples were collected using household cleaning cloths that were incubated overnight in buffered peptone water (BPW). Aliquots of BPW were then processed using two different selective enrichment and culture techniques. In the first technique (TBG-RV-XLT4) samples were incubated at 43 °C in tetrathionate broth and then Rappaport-Vassiliadis broth before plating on XLT4 agar. The second technique (SEL-XLD) involved incubation at 37 °C in selenite broth before plating on XLD agar. Salmonella was recovered from 49.7% (73/147) of samples using the TBG-RV-XLT4 technique, but only 10.2% (15/147) of samples using the SEL-XLD method. Fourteen samples (9.5%) were culture-positive using both methods, and 73 (49.7%) were culture-negative using both techniques. There were discordant results for 60 samples, including 59 that were only culture-positive using the TBG-RV-XLT4 method, and one sample that was only culturepositive using the SEL-XLD method. Salmonella was much more likely to be recovered using the TBG-RV-XLT4 method, and there appeared to be five times more false-negative results using the SEL-XLD technique. Environmental contamination with Salmonella may be underestimated by certain culture techniques, which may impair efforts to control spread in veterinary hospitals.
Details
- Title
- Comparison of two culture techniques used to detect environmental contamination with Salmonella enterica in a large-animal hospital
- Authors/Creators
- C.H. Lyle (Author/Creator) - University of PretoriaC.H. Annandale (Author/Creator) - University of PretoriaJ. Gouws (Author/Creator) - University of PretoriaP.S. Morley (Author/Creator) - University of South Africa
- Publication Details
- Journal of the South African Veterinary Association, Vol.86(1), a1292
- Publisher
- AOSIS
- Identifiers
- 991005540375107891
- Copyright
- © 2018 The Authors
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.42 Bacteriology
- 1.42.376 Salmonella and Campylobacter
- Web Of Science research areas
- Veterinary Sciences
- ESI research areas
- Plant & Animal Science