Complete development and long-term maintenance of Cryptosporidium parvum human and cattle genotypes in cell culture

N. Hijjawi; B.P. Meloni; U.M. Morgan; R.C.A. Thompson

doi:10.1016/S0020-7519(01)00212-0

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Complete development and long-term maintenance of Cryptosporidium parvum human and cattle genotypes in cell culture

Journal article

Peer reviewed

Complete development and long-term maintenance of Cryptosporidium parvum human and cattle genotypes in cell culture

N. Hijjawi, B.P. Meloni, U.M. Morgan and R.C.A. Thompson

International Journal for Parasitology, Vol.31(10), pp.1048-1055

2001

DOI: https://doi.org/10.1016/S0020-7519(01)00212-0

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Abstract

This study describes the complete development (from sporozoites to sporulated oocysts) of Cryptosporidium parvum (human and cattle genotypes) in the HCT-8 cell line. Furthermore, for the first time the complete life cycle was perpetuated in vitro for up to 25 days by subculturing. The long-term maintenance of the developmental cycle of the parasite in vitro appeared to be due to the initiation of the auto-reinfection cycle of C. parvum. This auto-reinfection is characterised by the production and excystation of new invasive sporozoites from thin-walled oocysts, with subsequent maintenance of the complete life cycle in vitro. In addition, thin-walled oocysts of the cattle genotype were infective for ARC/Swiss mice but similar oocysts of the human genotype were not. This culture system will provide a model for propagation of the complete life cycle of C. parvum in vitro.

Details

Title: Complete development and long-term maintenance of Cryptosporidium parvum human and cattle genotypes in cell culture
Authors/Creators: N. Hijjawi (Author/Creator) - Murdoch University
B.P. Meloni (Author/Creator) - Sir Charles Gairdner Hospital
U.M. Morgan (Author/Creator) - Murdoch University
R.C.A. Thompson (Author/Creator) - Murdoch University
Publication Details: International Journal for Parasitology, Vol.31(10), pp.1048-1055
Publisher: Elsevier BV
Identifiers: 991005542330007891
Copyright: © 2001 Australian Society for Parasitology Inc.
Murdoch Affiliation: School of Veterinary and Biomedical Sciences
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.246 Diarrheal Diseases; 1.246.985 Cryptosporidium
Web Of Science research areas: Parasitology
ESI research areas: Microbiology