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Journal article
Comprehensive Characterization of Long Non-Coding RNAs in Porcine Tissues: Expression Patterns and Functional Insights During Oocyte Development
Cells (Basel, Switzerland), Vol.14(18), 1409
2025
PMID: 41002376
Abstract
Long non-coding RNAs (lncRNAs) are essential regulatory molecules involved in various biological processes in mammals. However, their expression patterns across multiple porcine tissues have not been systematically characterized. We analyzed 607 RNA-seq datasets derived from 14 porcine tissues, including backfat, gallbladder, heart, ileum, jejunum, kidney, longissimus dorsi, liver, lung, skeletal muscle, ovary, pituitary, skeletal muscle, and spleen. Additionally, we examined 63 single-cell RNA-seq datasets from porcine primary oocytes at five developmental stages. For comparative analysis, we included 20 human and 17 mouse oocyte RNA-seq datasets. We identified 52,798 porcine lncRNAs, with tissue-specific expression patterns most prominent in oocytes and least in skeletal muscle. Among them, 2169 were classified as housekeeping and 14,469 as tissue-specific lncRNAs. Cross-species analysis revealed that a small subset of oocyte-expressed lncRNAs is conserved in humans and mice, associated with catalytic activity and circadian regulation. Additionally, 44 lncRNAs were differentially expressed during oocyte development, implicating them in neurogenesis, vesicle transport, and protein modification. Our findings not only contribute to the growing body of knowledge regarding lncRNAs in porcine biology but also pave the way for future research aimed at elucidating their functional roles in reproductive biology and other physiological processes.
Details
- Title
- Comprehensive Characterization of Long Non-Coding RNAs in Porcine Tissues: Expression Patterns and Functional Insights During Oocyte Development
- Authors/Creators
- Yao Jiang - Murdoch UniversityYipeng Li - South China Agricultural UniversityQingpeng Shen - South China Agricultural UniversityXiaolong Yuan - South China Agricultural UniversityFei Gao - Agricultural Genomics Institute at ShenzhenBin Ma - Murdoch University, School of Medical, Molecular and Forensic Sciences
- Publication Details
- Cells (Basel, Switzerland), Vol.14(18), 1409
- Publisher
- MDPI
- Number of pages
- 22
- Grant note
- 2024CXTD22 / the Modern Agricultural Industrial Technology System lnnovation Team of Guangdong Province NCTIP-XDB14 / the National Center of Technology Innovation for Pigs 2023-4408X1-29060200 / the Breed Industry Innovation Park of Guangdong Xiaoerhua Pig
- Identifiers
- 991005816849207891
- Copyright
- © 2025 by the authors.
- Murdoch Affiliation
- Centre for Healthy Ageing; School of Medical, Molecular and Forensic Sciences
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.196 Micro & Long Noncoding RNA
- 1.196.921 lncRNA
- Web Of Science research areas
- Cell Biology
- ESI research areas
- Biology & Biochemistry