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CC BY V4.0, Open Access
Journal article
Comprehensive transcriptome profiling uncovers molecular mechanisms and potential candidate genes associated with heat stress response in Chickpea
International journal of molecular sciences, Vol.24(2), Art. 1369
2023
PMID: 36674889
Abstract
Chickpea (Cicer arietinum L.) production is highly susceptible to heat stress (day/night temperatures above 32/20 degrees C). Identifying the molecular mechanisms and potential candidate genes underlying heat stress response is important for increasing chickpea productivity. Here, we used an RNA-seq approach to investigate the transcriptome dynamics of 48 samples which include the leaf and root tissues of six contrasting heat stress responsive chickpea genotypes at the vegetative and reproductive stages of plant development. A total of 14,544 unique, differentially expressed genes (DEGs) were identified across different combinations studied. These DEGs were mainly involved in metabolic processes, cell wall remodeling, calcium signaling, and photosynthesis. Pathway analysis revealed the enrichment of metabolic pathways, biosynthesis of secondary metabolites, and plant hormone signal transduction, under heat stress conditions. Furthermore, heat-responsive genes encoding bHLH, ERF, WRKY, and MYB transcription factors were differentially regulated in response to heat stress, and candidate genes underlying the quantitative trait loci (QTLs) for heat tolerance component traits, which showed differential gene expression across tolerant and sensitive genotypes, were identified. Our study provides an important resource for dissecting the role of candidate genes associated with heat stress response and also paves the way for developing climate-resilient chickpea varieties for the future.
Details
- Title
- Comprehensive transcriptome profiling uncovers molecular mechanisms and potential candidate genes associated with heat stress response in Chickpea
- Authors/Creators
- Himabindu Kudapa - International Crops Research Institute for the Semi-Arid TropicsRutwik Barmukh - SEMIVanika Garg - SEMIAnnapurna Chitikineni - SEMISrinivasan Samineni - SEMIGaurav Agarwal - Int Crops Res Inst Semi Arid Trop, Ctr Excellence Genom & Syst Biol, Patancheru 502324, IndiaRajeev K. K. Varshney - Murdoch University, Food Futures Institute
- Publication Details
- International journal of molecular sciences, Vol.24(2), Art. 1369
- Publisher
- MDPI
- Number of pages
- 20
- Grant note
- OPP1114827 / Bill and Melinda Gates Foundation through the Tropical Legumes III project Science & Engineering Research Board (SERB) of Department of Science and Technology (DST) Government of India
- Identifiers
- 991005564668807891
- Copyright
- © 2023 by the authors.
- Murdoch Affiliation
- Centre for Crop and Food Innovation; State Agricultural Biotechnology Centre
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.96 QTL
- Web Of Science research areas
- Biochemistry & Molecular Biology
- Chemistry, Multidisciplinary
- ESI research areas
- Chemistry