Concentrations of 15F2t isoprostane in urine of dogs with intervertebral disk disease

Maureen A McMichael; Craig G Ruaux; Wendy I Baltzer; Sharon C Kerwin; Giselle L Hosgood; Jörg M Steiner; David A Williams

doi:10.2460/ajvr.67.7.1226

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Concentrations of 15F2t isoprostane in urine of dogs with intervertebral disk disease

Journal article

Open access

Peer reviewed

Concentrations of 15F2t isoprostane in urine of dogs with intervertebral disk disease

Maureen A McMichael, Craig G Ruaux, Wendy I Baltzer, Sharon C Kerwin, Giselle L Hosgood, Jörg M Steiner and David A Williams

American journal of veterinary research, Vol.67(7), pp.1226-1231

2006

DOI: https://doi.org/10.2460/ajvr.67.7.1226

PMID: 16817747

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Abstract

Objective To measure 15F2t isoprostane concentrations in the urine of dogs undergoing ovariohysterectomy (OHE) and dogs undergoing surgery because of intervertebral disk disease (IVDD) and to assess relationships between urinary concentrations of 15F2t isoprostanes and neurologic score in dogs with IVDD. Animals 11 dogs undergoing OHE and 32 dogs with IVDD undergoing hemilaminectomy. Procedures Paired urine samples were obtained at induction of anesthesia and approximately 1 hour after OHE (controls) and were collected from dogs with IVDD at induction of anesthesia (28 samples) and approximately 1 hour after hemilaminectomy (31 samples); 26 paired urine samples were obtained from dogs with IVDD. Urinary isoprostane concentrations were measured by use of a commercial ELISA, and results were adjusted on the basis of urinary creatinine concentrations. Differences in the mean isoprostane-to-creatinine ratio were analyzed. Neurologic score was determined in dogs with IVDD by use of the modified Frankel scoring system. Results Urinary isoprostane-to-creatinine ratios were significantly higher in dogs with IVDD than in control dogs before and after surgery. There was no significant difference between values before and after surgery for either group. There was a significant correlation of neurologic score and urinary isoprostane-to-creatinine ratio because dogs that had higher neurologic scores (ie, less severely affected) generally had higher isoprostane-to-creatinine ratios. Conclusions and Clinical Relevance Urinary isoprostane-to-creatinine ratios were higher in dogs with IVDD before and after surgery. Analysis of these data suggests that dogs with IVDD are in a state of oxidative stress and that preemptive treatment with antioxidants warrants further investigation.

Details

Title: Concentrations of 15F2t isoprostane in urine of dogs with intervertebral disk disease
Authors/Creators: Maureen A McMichael - Texas A&M University
Craig G Ruaux - Oregon State University
Wendy I Baltzer - Oregon State University
Sharon C Kerwin - Mitchell Institute
Giselle L Hosgood - Louisiana State University
Jörg M Steiner - Mitchell Institute
David A Williams
Publication Details: American journal of veterinary research, Vol.67(7), pp.1226-1231
Identifiers: 991005592644207891
Murdoch Affiliation: School of Veterinary Medicine
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.68 Lipids; 1.68.707 Oxidative Lipid Damage
Web Of Science research areas: Veterinary Sciences
ESI research areas: Plant & Animal Science