Journal article
Construction of a promoter-rescue plasmid for butyrivibrio fibrisolvens and its use in characterization of a Flagellin promoter
Current Microbiology, Vol.40(3), pp.164-168
2000
Abstract
The Butyrivibrio fibrisolvens/Escherichia coli shuttle vector pBHerm has been modified to produce a plasmid (pBHE) that can be used for the identification and characterization of promoters in B. fibrisolvens. pBHE allows the insertion of a test promoter immediately upstream of a promoterless erythromycin resistance gene (ermAM). The efficacy of the pBHE plasmid in isolating and characterizing promoters was tested by inserting the flagellin gene (flaA) promoter from B. fibrisolvens OR77. Transcription of the ermAM gene from the flaA promoter was significantly higher than that observed when the ermAM gene was under the control of its own promoter. The flagelling gene of OR77 appears to be transcribed from two different promoters that produce transcripts initiating approximately 130 bp apart. Two mutant flaA promoter constructs, containing mutations in the −10 and −35 regions of either of the two putative promoter regions, showed drastic alterations in both the origin and amounts of the two transcripts produced. Mutations in either promoter affected transcription from both promoters, indicating that both regions contribute to gene expression.
Details
- Title
- Construction of a promoter-rescue plasmid for butyrivibrio fibrisolvens and its use in characterization of a Flagellin promoter
- Authors/Creators
- C.E. Beard (Author/Creator) - Commonwealth Scientific and Industrial Research OrganisationK. Gregg (Author/Creator) - Murdoch UniversityM. Kalmokoff (Author/Creator) - Health CanadaR.M. Teather (Author/Creator) - Agriculture and Agri-Food Canada
- Publication Details
- Current Microbiology, Vol.40(3), pp.164-168
- Publisher
- Springer Verlag
- Identifiers
- 991005540436407891
- Murdoch Affiliation
- School of Biological Sciences and Biotechnology
- Language
- English
- Resource Type
- Journal article
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- 3 Agriculture, Environment & Ecology
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- Web Of Science research areas
- Microbiology
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- Microbiology