Construction of genotyping-by-sequencing based high-density genetic maps and QTL mapping for fusarium wilt resistance in pigeonpea

R.K. Saxena; V.K. Singh; S.M. Kale; R. Tathineni; S. Parupalli; V. Kumar; V. Garg; R.R. Das; M. Sharma; K.N. Yamini; S. Muniswamy; A. Ghanta; A. Rathore; C.V.S. Kumar; K.B. Saxena; P.B.K. Kishor; R.K. Varshney

doi:10.1038/s41598-017-01537-2

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Construction of genotyping-by-sequencing based high-density genetic maps and QTL mapping for fusarium wilt resistance in pigeonpea

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Construction of genotyping-by-sequencing based high-density genetic maps and QTL mapping for fusarium wilt resistance in pigeonpea

R.K. Saxena, V.K. Singh, S.M. Kale, R. Tathineni, S. Parupalli, V. Kumar, V. Garg, R.R. Das, M. Sharma, K.N. Yamini, …

Scientific Reports, Vol.7(1), Art. 1911

2017

DOI: https://doi.org/10.1038/s41598-017-01537-2

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Abstract

Fusarium wilt (FW) is one of the most important biotic stresses causing yield losses in pigeonpea. Genetic improvement of pigeonpea through genomics-assisted breeding (GAB) is an economically feasible option for the development of high yielding FW resistant genotypes. In this context, two recombinant inbred lines (RILs) (ICPB 2049 × ICPL 99050 designated as PRIL_A and ICPL 20096 × ICPL 332 designated as PRIL_B) and one F2 (ICPL 85063 × ICPL 87119) populations were used for the development of high density genetic maps. Genotyping-by-sequencing (GBS) approach was used to identify and genotype SNPs in three mapping populations. As a result, three high density genetic maps with 964, 1101 and 557 SNPs with an average marker distance of 1.16, 0.84 and 2.60 cM were developed in PRIL_A, PRIL_B and F2, respectively. Based on the multi-location and multi-year phenotypic data of FW resistance a total of 14 quantitative trait loci (QTLs) including six major QTLs explaining >10% phenotypic variance explained (PVE) were identified. Comparative analysis across the populations has revealed three important QTLs (qFW11.1, qFW11.2 and qFW11.3) with upto 56.45% PVE for FW resistance. This is the first report of QTL mapping for FW resistance in pigeonpea and identified genomic region could be utilized in GAB.

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Title: Construction of genotyping-by-sequencing based high-density genetic maps and QTL mapping for fusarium wilt resistance in pigeonpea
Authors/Creators: R.K. Saxena (Author/Creator) - International Crops Research Institute for the Semi-Arid Tropics
V.K. Singh (Author/Creator) - International Crops Research Institute for the Semi-Arid Tropics
S.M. Kale (Author/Creator) - International Crops Research Institute for the Semi-Arid Tropics
R. Tathineni (Author/Creator) - International Crops Research Institute for the Semi-Arid Tropics
S. Parupalli (Author/Creator) - International Crops Research Institute for the Semi-Arid Tropics
V. Kumar (Author/Creator) - International Crops Research Institute for the Semi-Arid Tropics
V. Garg (Author/Creator) - International Crops Research Institute for the Semi-Arid Tropics
R.R. Das (Author/Creator) - International Crops Research Institute for the Semi-Arid Tropics
M. Sharma (Author/Creator) - International Crops Research Institute for the Semi-Arid Tropics
K.N. Yamini (Author/Creator) - Professor Jayashankar Telangana State Agricultural University
S. Muniswamy (Author/Creator) - University of Agricultural Sciences Raichur
A. Ghanta (Author/Creator) - Professor Jayashankar Telangana State Agricultural University
A. Rathore (Author/Creator) - International Crops Research Institute for the Semi-Arid Tropics
C.V.S. Kumar (Author/Creator) - International Crops Research Institute for the Semi-Arid Tropics
K.B. Saxena (Author/Creator) - International Crops Research Institute for the Semi-Arid Tropics
P.B.K. Kishor (Author/Creator) - Osmania University
R.K. Varshney (Author/Creator) - International Crops Research Institute for the Semi-Arid Tropics
Publication Details: Scientific Reports, Vol.7(1), Art. 1911
Publisher: Springer Nature
Identifiers: 991005544279207891
Copyright: © 2017 The Authors.
Murdoch Affiliation: Murdoch University; Centre for Crop and Food Innovation; State Agricultural Biotechnology Centre
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.4 Crop Science; 3.4.96 QTL
Web Of Science research areas: Plant Sciences
ESI research areas: Plant & Animal Science