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Continuous non-destructive hydrocarbon extraction from Botryococcus braunii BOT-22
Journal article   Peer reviewed

Continuous non-destructive hydrocarbon extraction from Botryococcus braunii BOT-22

P. Mehta, B.A. Jackson, E.G. Nwoba, A. Vadiveloo, P.A. Bahri, A.S. Mathur and N.R. Moheimani
Algal Research, Vol.41, Article number 101537
2019
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Abstract

In-situ extraction of microalgal oil without cell destruction has been proposed as a cost-effective alternative solution for the production of algal biofuel. This study examines the viability and efficiency of n-dodecane in both column and shake flask systems for the continuous extraction of botryococcene from Botryococcus braunii (BOT-22). Botryococcene from B. braunii was found to be non-destructively extracted using n-dodecane without negatively affecting the growth and photophysiology of the microalgae. Recirculation of the culture (23 times per day)through a column system containing n-dodecane with a daily effective extraction time of 276 s resulted in 21% higher botryococcene extraction (25.1 mg g −1 biomass)when compared to a constantly stirred shake flask consisting of both algae culture and n-dodecane together in a vessel. Botryococcene extraction was highest in the first 24 h of extraction when compared to the rest of the extraction period. These results suggest that recirculation of B. braunii culture through a column system containing n-dodecane is efficient for the non-destructive extraction of botryococcene and less toxic compared to other previously tested solvents.

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Collaboration types
Industry collaboration
Domestic collaboration
International collaboration
Citation topics
3 Agriculture, Environment & Ecology
3.171 Photoproductivity
3.171.477 Microalgae Biotechnology
Web Of Science research areas
Biotechnology & Applied Microbiology
ESI research areas
Biology & Biochemistry
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