Core Alzheimer’s disease cerebrospinal fluid biomarker assays are not affected by aspiration or gravity drip extraction methods

J.D. Doecke; C. Francois; C.J. Fowler; E. Stoops; P. Bourgeat; S.R. Rainey-Smith; Q-X Li; C.L. Masters; R.N. Martins; V.L. Villemagne; S.J. Collins; H.M. Vanderstichele

doi:10.1186/s13195-021-00812-9

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Core Alzheimer’s disease cerebrospinal fluid biomarker assays are not affected by aspiration or gravity drip extraction methods

Journal article

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Core Alzheimer’s disease cerebrospinal fluid biomarker assays are not affected by aspiration or gravity drip extraction methods

J.D. Doecke, C. Francois, C.J. Fowler, E. Stoops, P. Bourgeat, S.R. Rainey-Smith, Q-X Li, C.L. Masters, R.N. Martins, V.L. Villemagne, …

Alzheimer's Research & Therapy, Vol.13(1), Art. 79

2021

DOI: https://doi.org/10.1186/s13195-021-00812-9

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Abstract

Background CSF biomarkers are well-established for routine clinical use, yet a paucity of comparative assessment exists regarding CSF extraction methods during lumbar puncture. Here, we compare in detail biomarker profiles in CSF extracted using either gravity drip or aspiration. Methods Biomarkers for β-amyloidopathy (Aβ1–42, Aβ1–40), tauopathy (total tau), or synapse pathology (BACE1, Neurogranin Trunc-p75, α-synuclein) were assessed between gravity or aspiration extraction methods in a sub-population of the Australian Imaging, Biomarkers and Lifestyle (AIBL) study (cognitively normal, N = 36; mild cognitive impairment, N = 8; Alzheimer’s disease, N = 6). Results High biomarker concordance between extraction methods was seen (concordance correlation > 0.85). Passing Bablock regression defined low beta coefficients indicating high scalability. Conclusions Levels of these commonly assessed CSF biomarkers are not influenced by extraction method. Results of this study should be incorporated into new consensus guidelines for CSF collection, storage, and analysis of biomarkers.

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Title: Core Alzheimer’s disease cerebrospinal fluid biomarker assays are not affected by aspiration or gravity drip extraction methods
Authors/Creators: J.D. Doecke (Author/Creator) - Australian e-Health Research Centre
C. Francois (Author/Creator) - ADx NeuroSciences
C.J. Fowler (Author/Creator) - The University of Melbourne
E. Stoops (Author/Creator) - ADx NeuroSciences
P. Bourgeat (Author/Creator) - Australian e-Health Research Centre
S.R. Rainey-Smith (Author/Creator)
Q-X Li (Author/Creator) - The University of Melbourne
C.L. Masters (Author/Creator) - The University of Melbourne
R.N. Martins (Author/Creator) - Edith Cowan University
V.L. Villemagne (Author/Creator) - University of Pittsburgh
S.J. Collins (Author/Creator) - The University of Melbourne
H.M. Vanderstichele (Author/Creator) - Biomarkable, Ghent, Belgium
Publication Details: Alzheimer's Research & Therapy, Vol.13(1), Art. 79
Publisher: Biomed Central
Identifiers: 991005543984907891
Copyright: © 2021 The Authors.
Murdoch Affiliation: Centre for Healthy Ageing; Health Futures Institute
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.52 Neurodegenerative Diseases; 1.52.60 Dementia
Web Of Science research areas: Clinical Neurology; Neurosciences
ESI research areas: Neuroscience & Behavior