Journal article
Corneal biopsy with tissue micro homogenisation for isolation of organisms in bacterial keratitis
Eye, Vol.13(4), pp.545-549
1999
Abstract
Purpose To evaluate a novel two-stage technique to increase yield of bacteria isolated from infected corneal ulcers.
Methods A new blade was designed to remove friable material from infected corneal ulcers. The new blade was used in combination with standard tissue micro-homogenisation equipment in a two-stage technique intended to distribute biopsy samples evenly between relevant agar plates. Patients with presumed-bacterial corneal ulcers underwent sequential corneal sampling using the new two-stage technique and a scalpel blade, used without micro-homogenisation (the order of sampling was varied between two groups). Bacterial isolation rates were compared using the chi-squared test.
Results Twenty-four patients with presumed-bacterial corneal ulcers were studied. The overall positive bacterial isolation rate was 88%, with identical bacterial isolation rates for the new two-stage technique and the scalpel blade (71%). The new technique isolated bacteria from three ulcers that had initially been ‘sterile’ when sampled with a scalpel blade. Polymicrobial infections were identified in two ulcers with the new blade where only a single organism had been identified using the scalpel blade (not significantly different).
Conclusions The new two-stage technique shows promise for improving bacterial isolation rates from presumed-bacterial corneal ulcers.
Details
- Title
- Corneal biopsy with tissue micro homogenisation for isolation of organisms in bacterial keratitis
- Authors/Creators
- J. Diamond (Author/Creator) - Bristol Eye HospitalJ. Leeming (Author/Creator) - Bristol Royal InfirmaryG. Coombs (Author/Creator) - Royal Perth HospitalJ. Pearman (Author/Creator) - Royal Perth HospitalA. Sharma (Author/Creator) - Bristol Eye HospitalC. Illingworth (Author/Creator)G. Crawford (Author/Creator) - Lions Eye InstituteD. Easty (Author/Creator) - Bristol Eye Hospital
- Publication Details
- Eye, Vol.13(4), pp.545-549
- Publisher
- Nature Publishing Group
- Identifiers
- 991005543646507891
- Copyright
- © 1999 Royal College of Ophthalmologists
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.36 Ophthalmology
- 1.36.686 Ocular Surface Disorders
- Web Of Science research areas
- Ophthalmology
- ESI research areas
- Clinical Medicine