Files and links (1)
CC BY V4.0, Open Access
Journal article
Cross-platform mass spectrometry annotation in breathomics of oesophageal-gastric cancer
Scientific reports, Vol.8, 5139
2018
PMID: 29666423
Abstract
Disease breathomics is gaining importance nowadays due to its usefulness as non-invasive early cancer detection. Mass spectrometry (MS) technique is often used for analysis of volatile organic compounds (VOCs) associated with cancer in the exhaled breath but a long-standing challenge is the uncertainty in mass peak annotation for potential volatile biomarkers. This work describes a crossplatform MS strategy employing selected-ion flow tube mass spectrometry (SIFT-MS), high resolution gas chromatography-mass spectrometry (GC-MS) retrofitted with electron ionisation (EI) and GC-MS retrofitted with positive chemical ionisation (PCI) as orthogonal analytical approaches in order to provide facile identification of the oxygenated VOCs from breath of cancer patients. In addition, water infusion was applied as novel efficient PCI reagent in breathomics analysis, depicting unique diagnostic ions M+ or [ M-17](+) for VOC identification. Identity confirmation of breath VOCs was deduced using the proposed multi-platform workflow, which reveals variation in breath oxygenated VOC composition of oesophageal-gastric (OG) cancer patients with dominantly ketones, followed by aldehydes, alcohols, acids and phenols in decreasing order of relative abundance. Accurate VOC identification provided by cross-platform approach would be valuable for the refinement of diagnostic VOC models and the understanding of molecular drivers of VOC production.
Details
- Title
- Cross-platform mass spectrometry annotation in breathomics of oesophageal-gastric cancer
- Authors/Creators
- Sung-Tong Chin - Imperial Coll London, Div Surg, Dept Surg & Canc, London W2 1NY, EnglandAndrea Romano - Imperial College LondonSophie L. F. Doran - Imperial Coll London, Div Surg, Dept Surg & Canc, London W2 1NY, EnglandGeorge B Hanna - Imperial College London
- Publication Details
- Scientific reports, Vol.8, 5139
- Publisher
- NATURE PORTFOLIO
- Number of pages
- 10
- Grant note
- Rosetrees TrustStonygates TrustNational Institute for Health Research (NIHR) Diagnostic Evidence Co-operative London at Imperial College Healthcare NHS Trust
S.-T. Chin acknowledges the fellowship support from Rosetrees and Stonygates Trusts. The authors thank Eco Scientific and Mr Stephen Durrant for the loan of a permeation unit, as well as Professor Patrik Spanel and Professor David Smith for their helpful discussion. This research was supported by the Rosetrees and Stoneygate Trusts and the National Institute for Health Research (NIHR) Diagnostic Evidence Co-operative London at Imperial College Healthcare NHS Trust. The views expressed are those of the authors and not necessarily those of the NHS, the NIHR or the Department of Health.
- Identifiers
- 991005743544207891
- Copyright
- © The Author(s) 2018
- Murdoch Affiliation
- School of Medical, Molecular and Forensic Sciences
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Metrics
82 File views/ downloads
19 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.220 Smell & Taste Science
- 3.220.1595 Electronic Nose
- Web Of Science research areas
- Chemistry, Analytical
- ESI research areas
- Chemistry