Journal article
Crossmatching maternal and fetal blood in sheep
Comparative Medicine, Vol.68(4), pp.294-297
2018
Abstract
We investigated the incidence of ex vivo incompatibility between ovine maternal RBC and fetal plasma. Time-mated singleton pregnant ewes (n = 8) underwent cesarean delivery of the fetus; at the time of delivery, paired maternal and fetal blood samples were collected and subsequently separated for storage as packed RBC and fresh frozen plasma. Gel column crossmatching was performed 3 to 4 wk later. All fetus–dam crossmatches were considered major crossmatches, combining fetal (recipient) plasma with dam (donor) RBC. The plasma of 8 fetuses was cross-matched with RBC from 5 dams; all autologous controls were negative, and all but one crossmatch (1 of 40, 2.5%) were considered compatible. In addition, the plasma of 3 dams was crossmatched with RBC from 5 dams; all autologous controls were negative; however, significant incompatibility was noted. In total, 4 of 13 (30.8%) dam–dam crossmatches were considered incompatible. The results of this initial study suggest that when a single animal receives multiple blood-product transfusions, the risk of an immunologic transfusion reaction can be reduced by ensuring that the blood products are obtained from a single donor, performing a crossmatch prior to transfusion, and the use of synthetic products to increase the oxygen carrying capacity of fetal blood.
Details
- Title
- Crossmatching maternal and fetal blood in sheep
- Authors/Creators
- G.C. Musk (Author/Creator)M.J. Adams (Author/Creator)H. Usuda (Author/Creator)M.W. Kemp (Author/Creator)C.R. Sharp (Author/Creator)
- Publication Details
- Comparative Medicine, Vol.68(4), pp.294-297
- Publisher
- American Association for Laboratory Animal Science
- Identifiers
- 991005545532407891
- Copyright
- © 2018 American Association for Laboratory Animal Science
- Murdoch Affiliation
- School of Veterinary and Life Sciences
- Language
- English
- Resource Type
- Journal article
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- 10 Arts & Humanities
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- Web Of Science research areas
- Veterinary Sciences
- Zoology
- ESI research areas
- Clinical Medicine