Journal article
Csk-binding protein controls red blood cell development via regulation of Lyn tyrosine kinase activity
Experimental Hematology, Vol.46, pp.70-82.e10
2017
Abstract
Erythropoiesis is controlled principally through erythropoietin (Epo) receptor signaling, which involves Janus kinase 2 (JAK2) and Lyn tyrosine kinase, both of which are important for regulating red blood cell (RBC) development. Negative regulation of Lyn involves C-Src kinase (Csk)-mediated phosphorylation of its C-terminal tyrosine, which is facilitated by the transmembrane adaptor Csk-binding protein (Cbp). Although Cbp has significant functions in controlling Lyn levels and activity in erythroid cells in vitro, its importance to primary erythroid cell development and signaling has remained unclear. To address this, we assessed the consequence of loss of Cbp on the erythroid compartment in vivo and whether Epo-responsive cells isolated from Cbp-knockout mice exhibited altered signaling. Our data show that male Cbp−/− mice display a modest but significant alteration to late erythroid development in bone marrow with evidence of increased erythrocytes in the spleen, whereas female Cbp−/− mice exhibit a moderate elevation in early erythroid progenitors (not seen in male mice) that does not influence the later steps in RBC development. In isolated primary erythroid cells and cell lines generated from Cbp−/− mice, survival signaling through Lyn/Akt/FoxO3 was elevated, resulting in sustained viability during differentiation. The high Akt activity disrupted GAB2/SHP-2 feedback inhibition of Lyn; however, the elevated Lyn activity also increased inhibitory signaling via SHP-1 to restrict the Erk1/2 pathway. Interestingly, whereas loss of Cbp led to mild changes to late RBC development in male mice, this was not apparent in female Cbp−/− mice, possibly due to their elevated estrogen, which is known to facilitate early progenitor self-renewal.
Details
- Title
- Csk-binding protein controls red blood cell development via regulation of Lyn tyrosine kinase activity
- Authors/Creators
- J.H.C. Plani-Lam (Author/Creator) - Harry Perkins Institute of Medical ResearchN.S. Slavova-Azmanova (Author/Creator) - Harry Perkins Institute of Medical ResearchN. Kucera (Author/Creator) - Harry Perkins Institute of Medical ResearchA. Louw (Author/Creator) - Harry Perkins Institute of Medical ResearchJ. Satiaputra (Author/Creator) - Harry Perkins Institute of Medical ResearchP. Singer (Author/Creator) - Bioprocessing Technology InstituteK-P Lam (Author/Creator) - Bioprocessing Technology InstituteM. L. Hibbs (Author/Creator) - Monash UniversityE. Ingley (Author/Creator) - Harry Perkins Institute of Medical Research
- Publication Details
- Experimental Hematology, Vol.46, pp.70-82.e10
- Publisher
- Elsevier Inc.
- Identifiers
- 991005544243607891
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.184 Physiology & Metals
- 1.184.1030 Erythropoietin Therapy
- Web Of Science research areas
- Hematology
- Medicine, Research & Experimental
- ESI research areas
- Clinical Medicine