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Journal article
Current situation and genomic characterization of fowl pox virus in lower Egypt during 2022
Poultry science, Vol.102(8), 102769
2023
Abstract
A total of 45 samples of vaccinated and non-vaccinated layer chickens were collected from farms in the Egyptian governorates of Sharqia, Ismailia, Menofia, Gharbia, Kafr El Sheikh, Qalyubia, and Dakahlia in the year 2022. They exhibited nodular lesions on their combs, mouth corners, and eyelids, suggesting they were infected with pox disease, which was associated with a 3 to 5% mortality rate. The samples were grown on the chorioallantoic-membrane of embryonated chicken eggs to ensure their viability. In both vaccinated and non-vaccinated farms, 35 of 45 virus isolates were confirmed positive via polymerase chain reaction (PCR) of fpv167 (P4b), based on the amplicon length of the fpv167 gene locus. The 6 strains from various Egyptian governorates were chosen for sequencing and genetic characterization. Phylogenetic investigation of the fpv167 (P4b) gene of sequenced strains clustered within sub clade A1 showed 100% correlation between FWPVD, TKPV13401 and fowlpox-AN2, fowlpox-AN3, and fowlpox-AN6, but only a 98.6% correlation between fowlpox-AN1, fowlpox-AN4, and fowlpox-AN5. Comparing the fowlpox-AN1, fowlpox-AN4, and fowlpox-AN5 strains with commercial vaccine strains (HP1-444-(FP9), vaccine-VSVRI), they had 98.6% identity, while other strains had 100% identity. The results of this study's mutation research showed that fowlpox-AN1, fowlpox-AN4, and fowlpox-AN5 had acquired novel mutations; fowlpox-AN1 had R201G and T204A; fowlpox-AN4 and fowlpox-AN5 had L141F and H157P. Further research is required to determine the effectiveness of the current vaccine in order to develop a new vaccine.
Details
- Title
- Current situation and genomic characterization of fowl pox virus in lower Egypt during 2022
- Authors/Creators
- Nahed YehiaSabry ElsayedFatimah A. Al-SaeedAhmed Ezzat AhmedKhaled A. El-TarabilyMohamed T. El-SaadonyAhmed Abdelhalim
- Publication Details
- Poultry science, Vol.102(8), 102769
- Publisher
- Elsevier Inc. on behalf of Poultry Science Association Inc.
- Number of pages
- 8
- Grant note
- Abu Dhabi Educa-tion and Knowledge, UAE: 21S105 King Khalid University, Saudi Arabia: R.G.P2 161-44
Khaled El-Tarabily is grateful to Abu Dhabi Education and Knowledge (Grant #: 21S105), UAE for funding the current work. All authors are also grateful to the deanship of King Khalid University, Saudi Arabia for supporting this work under the fund number (R.G.P2 161-44) .
- Identifiers
- 991005580014207891
- Copyright
- © 2023 The Authors.
- Murdoch Affiliation
- Centre for Terrestrial Ecosystem Science and Sustainability
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.161 Virology - Identification & Sequencing
- 1.161.1662 Poxvirus Immunology
- Web Of Science research areas
- Agriculture, Dairy & Animal Science
- ESI research areas
- Plant & Animal Science