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Journal article
Current situation and genomic characterization of fowlpox virus in lower Egypt during 2022
Poultry science, Vol.102(8), 102769
2023
Abstract
A total of 45 samples of vaccinated and non-vaccinated layer chickens were collected from farms in the Egyptian governorates of Sharqia, Ismailia, Menofia, Gharbia, Kafr El Sheikh, Qalyubia, and Dakahlia in the year 2022. They exhibited nodular lesions on their combs, mouth corners, and eyelids, suggesting they were infected with pox disease, which was associated with a 3 to 5% mortality rate. The samples were grown on the chorioallantoic-membrane of embryonated chicken eggs to ensure their viability. In both vaccinated and non-vaccinated farms, 35 of 45 virus isolates were confirmed positive via polymerase chain reaction (PCR) of fpv167 (P4b), based on the amplicon length of the fpv167 gene locus. The 6 strains from various Egyptian governorates were chosen for sequencing and genetic characterization. Phylogenetic investigation of the fpv167 (P4b) gene of sequenced strains clustered within sub clade A1 showed 100% correlation between FWPVD, TKPV13401 and fowlpox-AN2, fowlpox-AN3, and fowlpox-AN6, but only a 98.6% correlation between fowlpox-AN1, fowlpox-AN4, and fowlpox-AN5. Comparing the fowlpox-AN1, fowlpox-AN4, and fowlpox-AN5 strains with commercial vaccine strains (HP1-444-(FP9), vaccine-VSVRI), they had 98.6% identity, while other strains had 100% identity. The results of this study's mutation research showed that fowlpox-AN1, fowlpox-AN4, and fowlpox-AN5 had acquired novel mutations; fowlpox-AN1 had R201G and T204A; fowlpox-AN4 and fowlpox-AN5 had L141F and H157P. Further research is required to determine the effectiveness of the current vaccine in order to develop a new vaccine.
Details
- Title
- Current situation and genomic characterization of fowlpox virus in lower Egypt during 2022
- Authors/Creators
- Nahed Yehia - Agricultural Research CenterSabry Elsayed - Reference Laboratory for Veterinary Quality Control on Poultry Production (RLQP), Animal Health Research Institute, Agriculture Research Center (ARC), Giza, 12618, EgyptFatimah A. Al-Saeed - Department of Biology, College of Science, King Khalid University, Abha, 61421, Saudi ArabiaAhmed Ezzat Ahmed - King Khalid UniversityKhaled A. El-Tarabily - Murdoch University, Centre for Terrestrial Ecosystem Science and SustainabilityMohamed T. El-Saadony - Zagazig UniversityAhmed Abd El-Halem Mohammed - Reference Laboratory for Veterinary Quality Control on Poultry Production (RLQP), Animal Health Research Institute, Agriculture Research Center (ARC), Giza, 12618, Egypt
- Publication Details
- Poultry science, Vol.102(8), 102769
- Publisher
- Elsevier Inc. on behalf of Poultry Science Association Inc.
- Identifiers
- 991005585959207891
- Copyright
- © 2023 The Authors.
- Murdoch Affiliation
- Harry Butler Institute
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.161 Virology - Identification & Sequencing
- 1.161.1662 Poxvirus Immunology
- Web Of Science research areas
- Agriculture, Dairy & Animal Science
- ESI research areas
- Plant & Animal Science