Cytoplasmic male sterility-associated chimeric open reading frames identified by mitochondrial genome sequencing of four cajanus genotypes

R. Tuteja; R.K. Saxena; J. Davila; T. Shah; W. Chen; Y-L Xiao; G. Fan; K.B. Saxena; A.J. Alverson; C. Spillane; C. Town; R.K. Varshney

doi:10.1093/dnares/dst025

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Cytoplasmic male sterility-associated chimeric open reading frames identified by mitochondrial genome sequencing of four cajanus genotypes

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Cytoplasmic male sterility-associated chimeric open reading frames identified by mitochondrial genome sequencing of four cajanus genotypes

R. Tuteja, R.K. Saxena, J. Davila, T. Shah, W. Chen, Y-L Xiao, G. Fan, K.B. Saxena, A.J. Alverson, C. Spillane, …

DNA Research, Vol.20(5), pp.485-495

2013

DOI: https://doi.org/10.1093/dnares/dst025

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Abstract

The hybrid pigeonpea (Cajanus cajan) breeding technology based on cytoplasmic male sterility (CMS) is currently unique among legumes and displays major potential for yield increase. CMS is defined as a condition in which a plant is unable to produce functional pollen grains. The novel chimeric open reading frames (ORFs) produced as a results of mitochondrial genome rearrangements are considered to be the main cause of CMS. To identify these CMS-related ORFs in pigeonpea, we sequenced the mitochondrial genomes of three C. cajan lines (the male-sterile line ICPA 2039, the maintainer line ICPB 2039, and the hybrid line ICPH 2433) and of the wild relative (Cajanus cajanifolius ICPW 29). A single, circular-mapping molecule of length 545.7 kb was assembled and annotated for the ICPA 2039 line. Sequence annotation predicted 51 genes, including 34 protein-coding and 17 RNA genes. Comparison of the mitochondrial genomes from different Cajanus genotypes identified 31 ORFs, which differ between lines within which CMS is present or absent. Among these chimeric ORFs, 13 were identified by comparison of the related male-sterile and maintainer lines. These ORFs display features that are known to trigger CMS in other plant species and to represent the most promising candidates for CMS-related mitochondrial rearrangements in pigeonpea.

Details

Title: Cytoplasmic male sterility-associated chimeric open reading frames identified by mitochondrial genome sequencing of four cajanus genotypes
Authors/Creators: R. Tuteja (Author/Creator) - International Crops Research Institute for the Semi-Arid Tropics
R.K. Saxena (Author/Creator) - SEMI
J. Davila (Author/Creator) - University of Nebraska–Lincoln
T. Shah (Author/Creator) - SEMI
W. Chen (Author/Creator) - BGI Group (China)
Y-L Xiao (Author/Creator) - J. Craig Venter Institute
G. Fan (Author/Creator) - BGI Group (China)
K.B. Saxena (Author/Creator) - SEMI
A.J. Alverson (Author/Creator) - University of Arkansas at Fayetteville
C. Spillane (Author/Creator) - Ollscoil na Gaillimhe – University of Galway
C. Town (Author/Creator) - J. Craig Venter Institute
R.K. Varshney (Author/Creator) - SEMI
Publication Details: DNA Research, Vol.20(5), pp.485-495
Publisher: Oxford University Press
Identifiers: 991005541051007891
Copyright: © 2013 The Authors.
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.4 Crop Science; 3.4.1922 Alternative Oxidase
Web Of Science research areas: Genetics & Heredity
ESI research areas: Molecular Biology & Genetics