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CC BY V4.0, Open Access
Journal article
Cytotoxicity of Methanol and Aqueous Olive Pomace Extracts Towards Pancreatic Cancer Cells In Vitro
Journal of Natural Products in Cancer Prevention and Therapy, Vol.2(1), pp.1-6
2018
Abstract
Pancreatic cancer is a devastating disease with a 5-year survival rate of less than 8%, and as such the search for novel treatments is vital. Olive pomace contains phenolic compounds which have displayed anti-cancer properties, however, the anti-pancreatic cancer potential of crude olive pomace extracts has yet to be determined. Therefore, the phenolic compound content and the cytotoxic activity of olive pomace extracts was determined in vitro. Moreover, the efficacy of water as an extraction solvent for the recovery of phenolic compounds from olive pomace was determined. Incubation with methanol pomace extract (100 µg/mL) for 72 hours caused growth inhibition in all cells tested; HT29 (colon) 35%, A2780 (ovarian) 53%, H460 (lung) 22%, A431 (skin) 38%, Du145 (prostate) 32%, BE2-C (neuroblastoma) 31%, MCF-7 (breast) 57%, and U87, SJ-G2, SMA (glioblastoma) 49%, 43% and 46% respectively, and one noncancer derived cell line MCF-10A (normal breast) 36% growth inhibition and reduced the viability of pancreatic cancer cells in a dose-dependent manner. Low concentrations (50 µg/mL) of methanol pomace extract increased the viability of non-tumorigenic cells (HPDE) compared to vehicle controls (20% increase). Additionally, the methanol extract contained a higher concentration of total phenolic compounds compared to the water extract (63.34 ± 4.82 and 51.55 ± 4.23 µg tyrosol equivalents/mL, respectively). Further work on the cytotoxicity of olive pomace extracts towards pancreatic cancer cells, and the protective effect on non-tumorigenic cells is warranted.
Details
- Title
- Cytotoxicity of Methanol and Aqueous Olive Pomace Extracts Towards Pancreatic Cancer Cells In Vitro
- Authors/Creators
- Chloe D. Goldsmith - Supreme Council Of HealthJennette SakoffCostas Stathopoulos - Murdoch University, Food Futures InstitutePaul D. Roach - Hunter WaterChristopher J. Scarlett - Hunter Water
- Publication Details
- Journal of Natural Products in Cancer Prevention and Therapy, Vol.2(1), pp.1-6
- Publisher
- United Scientific Group
- Identifiers
- 991005714569107891
- Copyright
- © 2018 Goldsmith et al.
- Murdoch Affiliation
- Food Futures Institute
- Resource Type
- Journal article
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