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CC BY V4.0, Open Access
Journal article
DNA metabarcoding reveals high relative abundance of trunk disease fungi in grapevines from Marlborough, New Zealand
BMC microbiology, Vol.22(1), Art. 126
2022
PMID: 35538413
Abstract
Grapevine trunk diseases (GTDs) are a threat to grape production worldwide, with a diverse collection of fungal species implicated in disease onset. Due to the long-term and complex nature of GTDs, simultaneous detection of multiple microbial species can enhance understanding of disease development. We used DNA metabarcoding of ribosomal internal transcribed spacer 1 (ITS1) sequences, supported by specific PCR and microbial isolation, to establish the presence of trunk pathogens across 11 vineyards (11-26 years old) over three years in Marlborough, the largest wine producing region in New Zealand. Using a reference database of trunk pathogen sequences, species previously associated with GTD, such as Cadophora luteo-olivacea, Diplodia seriata, Diplodia mutila, Neofusicoccum australe, and Seimatosporium vitis, were identified as highly represented across the vineyard region. The well-known pathogens Phaeomoniella chlamydospora and Eutypa lata had especially high relative abundance across the dataset, with P. chlamydospora reads present between 22 and 84% (average 52%) across the vineyards. Screening of sequences against broader, publicly available databases revealed further fungal species within families and orders known to contain pathogens, many of which appeared to be endemic to New Zealand. The presence of several wood-rotting basidiomycetes (mostly Hymenochaetales) was detected for the first time in the Marlborough vineyard region, notably, the native Inonotus nothofagii which was present at 1-2% relative abundance in two vineyards.
Details
- Title
- DNA metabarcoding reveals high relative abundance of trunk disease fungi in grapevines from Marlborough, New Zealand
- Authors/Creators
- Bhanupratap R Vanga - Canterbury Agriculture and Science Centre, The New Zealand Institute for Plant and Food Research Limited, Gerald St, Lincoln, 7608, New ZealandPreeti Panda - Canterbury Agriculture and Science Centre, The New Zealand Institute for Plant and Food Research Limited, Gerald St, Lincoln, 7608, New ZealandAnish ShahSarah Thompson - Canterbury Agriculture and Science Centre, The New Zealand Institute for Plant and Food Research Limited, Gerald St, Lincoln, 7608, New ZealandRebecca H Woolley - Marlborough Wine Research Centre, The New Zealand Institute for Plant and Food Research Limited, PO Box 845, Blenheim, New ZealandHayley J. Ridgway - Department of Pest Management and Conservation, Faculty of Agriculture and Life Sciences, Lincoln University, P O Box 84, Lincoln, 7647, New ZealandDion C Mundy - Marlborough Wine Research Centre, The New Zealand Institute for Plant and Food Research Limited, PO Box 845, Blenheim, New ZealandS. Bulman - Better Border Biosecurity (B3), Lincoln, 7608, New Zealand. simon.bulman@plantandfood.co.nz
- Publication Details
- BMC microbiology, Vol.22(1), Art. 126
- Identifiers
- 991005565169907891
- Copyright
- © 2022 The Author(s).
- Murdoch Affiliation
- School of Agricultural Sciences
- Language
- English
- Resource Type
- Journal article
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InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.97 Plant Pathology
- 3.97.1173 Hyphomycetes
- Web Of Science research areas
- Microbiology
- ESI research areas
- Microbiology