Journal article
Deciphering transcriptional programming during pod and seed development using RNA-Seq in Pigeonpea (Cajanus cajan)
PLoS ONE, Vol.11(10), Art. e0164959
2016
Abstract
Seed development is an important event in plant life cycle that has interested humankind since ages, especially in crops of economic importance. Pigeonpea is an important grain legume of the semi-arid tropics, used mainly for its protein rich seeds. In order to understand the transcriptional programming during the pod and seed development, RNA-seq data was generated from embryo sac from the day of anthesis (0 DAA), seed and pod wall (5, 10, 20 and 30 DAA) of pigeonpea variety “Asha” (ICPL 87119) using Illumina HiSeq 2500. About 684 million sequencing reads have been generated from nine samples, which resulted in the identification of 27,441 expressed genes after sequence analysis. These genes have been studied for their differentially expression, co-expression, temporal and spatial gene expression. We have also used the RNA-seq data to identify important seed-specific transcription factors, biological processes and associated pathways during seed development process in pigeonpea. The comprehensive gene expression study from flowering to mature pod development in pigeonpea would be crucial in identifying candidate genes involved in seed traits directly or indirectly related to yield and quality. The dataset will serve as an important resource for gene discovery and deciphering the molecular mechanisms underlying various seed related traits.
Details
- Title
- Deciphering transcriptional programming during pod and seed development using RNA-Seq in Pigeonpea (Cajanus cajan)
- Authors/Creators
- S.K. Parida (Author/Creator)L.T. Pazhamala (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsG. Agarwal (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsP. Bajaj (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsV. Kumar (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsA. Kulshreshtha (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsR.K. Saxena (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsR.K. Varshney (Author/Creator) - International Crops Research Institute for the Semi-Arid Tropics
- Publication Details
- PLoS ONE, Vol.11(10), Art. e0164959
- Publisher
- Public Library of Science
- Identifiers
- 991005542310707891
- Copyright
- © 2016 The Authors.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 1 Clinical & Life Sciences
- 1.54 Molecular & Cell Biology - Genetics
- 1.54.1543 Flow Cytometry
- Web Of Science research areas
- Plant Sciences
- ESI research areas
- Plant & Animal Science