Journal article
Deep sequencing of the fruit transcriptome and lipid accumulation in a non-seed tissue of Chinese tallow, a potential biofuel crop
Plant and Cell Physiology, Vol.57(1), pp.125-137
2015
Abstract
Chinese tallow (Triadica sebifera) is a valuable oilseedproducing tree that can grow in a variety of conditions without competing for food production, and is a promising biofuel feedstock candidate. The fruits are unique in that they contain both saturated and unsaturated fat present in the tallow and seed layer, respectively. The tallow layer is poorly studied and is considered only as an external fatty deposition secreted from the seed. In this study we show that tallow is in fact a non-seed cellular tissue capable of triglyceride synthesis. Knowledge of lipid synthesis and storage mechanisms in tissues other than seed is limited but essential to generate oil-rich biomass crops. Here, we describe the annotated transcriptome assembly generated from the fruit coat, tallow and seed tissues of Chinese tallow. The final assembly was functionally annotated, allowing for the identification of candidate genes and reconstruction of lipid pathways. A tallow tissue-specific paralog for the transcription factor gene WRINKLED1 (WRI1) and lipid droplet-associated protein genes, distinct from those expressed in seed tissue, were found to be active in tallow, underpinning the mode of oil synthesis and packaging in this tissue. Our data have established an excellent knowledge base that can provide genetic and biochemical insights for engineering non-seed tissues to accumulate large amounts of oil. In addition to the large data set of annotated transcripts, the study also provides gene-based simple sequence repeat and single nucleotide polymorphism markers.
Details
- Title
- Deep sequencing of the fruit transcriptome and lipid accumulation in a non-seed tissue of Chinese tallow, a potential biofuel crop
- Authors/Creators
- U.K. Divi (Author/Creator) - ACTX-R Zhou (Author/Creator) - Commonwealth Scientific and Industrial Research OrganisationP. Wang (Author/Creator) - Murdoch UniversityJ. Butlin (Author/Creator) - Commonwealth Scientific and Industrial Research OrganisationD-M Zhang (Author/Creator) - Shanghai Institute of Landscape GardeningQ. Liu (Author/Creator) - Commonwealth Scientific and Industrial Research OrganisationT. Vanhercke (Author/Creator) - Commonwealth Scientific and Industrial Research OrganisationJ.R. Petrie (Author/Creator) - Commonwealth Scientific and Industrial Research OrganisationM. Talbot (Author/Creator) - Commonwealth Scientific and Industrial Research OrganisationR.G. White (Author/Creator) - Commonwealth Scientific and Industrial Research OrganisationJ.M. Taylor (Author/Creator) - Commonwealth Scientific and Industrial Research OrganisationP. Larkin (Author/Creator) - Commonwealth Scientific and Industrial Research OrganisationS.P. Singh (Author/Creator) - Commonwealth Scientific and Industrial Research Organisation
- Publication Details
- Plant and Cell Physiology, Vol.57(1), pp.125-137
- Publisher
- Oxford University Press
- Identifiers
- 991005544300307891
- Copyright
- © The Author 2015.
- Murdoch Affiliation
- School of Veterinary and Life Sciences
- Language
- English
- Resource Type
- Journal article
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Source: InCites
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InCites Highlights
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.68 Lipids
- 1.68.1167 Lipid Biosynthesis Mechanisms
- Web Of Science research areas
- Cell Biology
- Plant Sciences
- ESI research areas
- Plant & Animal Science