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Derivation of two induced pluripotent stem cell lines from a healthy control subject
   

Derivation of two induced pluripotent stem cell lines from a healthy control subject

Dan Zhang, Di Huang, Leon M Larcher, Khine Zaw, Shang-Chih Chen, Luke Jennings, Tina M Lamey, Jennifer A Thompson, Terri L McLaren, Fred K Chen, …
Stem cell research, Vol.82, 103621
2025
: 39642595

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pluripotent stem3.87 MB
Open Access
Cell Differentiation Cell Line Cellular Reprogramming Embryoid Bodies - cytology Embryoid Bodies - metabolism Fibroblasts - cytology Fibroblasts - metabolism Humans Induced Pluripotent Stem Cells - cytology Induced Pluripotent Stem Cells - metabolism Kruppel-Like Factor 4 Male
Two human induced pluripotent stem cell lines, LEIi021-A and LEIi021-B, were derived from dermal fibroblasts from a healthy control subject from an Australian Aboriginal family with retinitis pigmentosa-11. Reprogramming was performed using episomal vectors expressing OCT4, SOX2, LIN28, L-MYC, KLF4 and mp53DD. Pluripotency markers were expressed in both LEIi021-A and LEIi021-B lines. The two cell lines displayed normal karyotypes and demonstrated the ability to differentiate into embryoid bodies with the three primary germ layers and retinal pigment epithelial cells.

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