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CC BY V4.0, Open Access
Journal article
Deriving fibroblast cell lines from wing-punch biopsies of Australian eastern bent-winged bats ( Miniopterus orianae oceanensis )
PeerJ (San Francisco, CA), Vol.13, e20222
2025
PMID: 41146999
Abstract
Cell cultures are a valuable tool for the study of in vitro disease processes, especially where such processes concern wild and/or threatened animal species. However, the collection of adequate samples for cell line preparation can be challenging under field conditions due to logistical challenges and access to equipment. In this paper, we describe the generation of fibroblast cell lines derived from wing-punch biopsies of Australian eastern bent-winged bats (
), expanding on and modifying existing protocols. Twenty wing-punch biopsies were collected from free-ranging individuals in New South Wales in February 2024 and shipped to the University of Melbourne, Victoria, within 24 hours. To assess the impact of different preservation methods on sample integrity, samples were subjected to two different shipping treatments: Ten were snap-frozen immediately upon collection, and the other ten were placed in cool phosphate-buffered saline (PBS) for transport. To test the effect of different plating treatments, samples were plated as either collagenase-digested cells or explants. Although none of the frozen biopsies or explants showed any growth, all samples transported in cool PBS and plated as digested cells remained viable. While agitation of the samples prior to plating resulted in an initially faster rate of cell growth, cells derived from tissue that had been digested multiple times spread across the plate and formed a monolayer significantly faster than those that had been digested only once. This study confirms the effectiveness of existing cell culture protocols using non-lethal sampling techniques in an Australian insectivorous bat species and shows a novel method of maximizing cell yield from a single biopsy. It also introduces an alternative transportation method, beneficial for field sample collection. These cell cultures are essential tools for future studies on disease susceptibility and pathogen responses in bat species, particularly those belonging to the family Miniopteridae. Additionally, they can be used for biobanking efforts, preserving the genetic material of non-model organisms for broader conservation purposes.
Details
- Title
- Deriving fibroblast cell lines from wing-punch biopsies of Australian eastern bent-winged bats ( Miniopterus orianae oceanensis )
- Authors/Creators
- Anna Langguth - The University of MelbourneLaura A Brannelly - The University of MelbourneChristopher Turbill - Western Sydney UniversityTomás Villada-Cadavid - Western Sydney UniversityNicholas C Wu - Murdoch University, School of Environmental and Conservation SciencesJasmin Hufschmid - The University of MelbourneEllen Cottingham - The University of Melbourne
- Publication Details
- PeerJ (San Francisco, CA), Vol.13, e20222
- Publisher
- Peerj Inc.
- Number of pages
- 20
- Identifiers
- 991005830346907891
- Copyright
- © 2025 Langguth et al.
- Murdoch Affiliation
- Centre for Terrestrial Ecosystem Science and Sustainability; School of Environmental and Conservation Sciences
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.35 Zoology & Animal Ecology
- 3.35.1587 Chiroptera
- Web Of Science research areas
- Multidisciplinary Sciences
- ESI research areas
- Plant & Animal Science