Detecting and measuring small numbers of viable Coxiella burnetii

M. Lockhart; A. Islam; S. Graves; S. Fenwick; J. Stenos

doi:10.1111/j.1574-695X.2011.00898.x

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Detecting and measuring small numbers of viable Coxiella burnetii

Journal article

Peer reviewed

Detecting and measuring small numbers of viable Coxiella burnetii

M. Lockhart, A. Islam, S. Graves, S. Fenwick and J. Stenos

FEMS Immunology & Medical Microbiology, Vol.64(1), pp.61-65

2012

DOI: https://doi.org/10.1111/j.1574-695X.2011.00898.x

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Abstract

Coxiella burnetii is an acidophilic, intracellular bacterium that causes the human disease Q fever. In some studies, it is important to distinguish between viable and nonviable C. burnetii. We compared four methods for detecting and measuring viable C. burnetii in biological samples as follows: growth in two different cell culture lines, infection of severe combined immunodeficient (SCID) mice (leading to death) and infection of SCID mice with detection of C. burnetii in their spleen (after euthanasia at day 50 postinfection). Two isolates of C. burnetii were used (Henzerling and Arandale). Our in-house qPCR assay for C. burnetii DNA was used as a control. SCID mouse inoculation was more sensitive than cell culture. The assay that detected C. burnetii in SCID mouse spleens was slightly more sensitive than SCID mice deaths alone. Approximately one viable C. burnetii cell could be detected by this method, making it suitable for determining the viability of C. burnetii in a sample.

Details

Title: Detecting and measuring small numbers of viable Coxiella burnetii
Authors/Creators: M. Lockhart (Author/Creator) - Geelong Football Club
A. Islam (Author/Creator) - New South Wales Department of Health
S. Graves (Author/Creator) - New South Wales Department of Health
S. Fenwick (Author/Creator) - Murdoch University
J. Stenos (Author/Creator) - Geelong Football Club
Publication Details: FEMS Immunology & Medical Microbiology, Vol.64(1), pp.61-65
Publisher: Wiley-Blackwell
Identifiers: 991005545456307891
Copyright: © 2011 Federation of European Microbiological Societies.
Murdoch Affiliation: School of Veterinary and Biomedical Sciences
Language: English
Resource Type: Journal article

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Citation topics: 1 Clinical & Life Sciences; 1.258 Zoonotic Diseases; 1.258.2263 Coxiella Burnetii
Web Of Science research areas: Immunology; Infectious Diseases; Microbiology
ESI research areas: Immunology