Detecting embryo developmental potential by single Blastomere RNA-Seq

Monika Nõmm; Marilin Ivask; Pille Pärn; Ene Reimann; Sulev Kõks; Ülle Jaakma

doi:10.3390/genes14030569

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Detecting embryo developmental potential by single Blastomere RNA-Seq

Journal article

Open access

Peer reviewed

Detecting embryo developmental potential by single Blastomere RNA-Seq

Monika Nõmm, Marilin Ivask, Pille Pärn, Ene Reimann, Sulev Kõks and Ülle Jaakma

Genes, Vol.14(3), Art. 569

2023

DOI: https://doi.org/10.3390/genes14030569

PMID: 36980841

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Abstract

embryo developmental potential

preimplantation embryo diagnostics

preimplantation embryo selection

single blastomere biopsy

single cell embryo biopsy

Recent advances in preimplantation embryo diagnostics enable a wide range of applications using single cell biopsy and molecular-based selection techniques without compromising embryo production. This study was conducted to develop a single cell embryo biopsy technique and gene expression analysis method with a very low input volume to ensure normal embryo development and to see if there are differences in gene expression profiles between day-5 biopsied bovine embryos that developed into blastocysts and embryos arrested at morula stage. Out of the 65 biopsied morulae, 32 developed to blastocysts (49.2%). Out of the 13,580 successfully annotated genes, 1204 showed a difference in mRNA expression level. Out of these, 155 genes were expressed in embryos developing to blastocysts. The pathway enrichment analysis revealed significant enrichment in “organelle biogenesis and maintenance”, “mRNA splicing” and “mitochondrial translation” pathways. These findings suggest principal differences in gene expression patterns and functional networks of embryos able to reach the blastocyst stage compared to embryos arrested in development. Our preliminary data suggest that single blastomere biopsy and selected gene expression profiles at morula stage could offer additional possibilities for early preimplantation embryo selection before transfer.

Details

Title: Detecting embryo developmental potential by single Blastomere RNA-Seq
Authors/Creators: Monika Nõmm - Estonian University of Life Sciences
Marilin Ivask - Estonian University of Life Sciences
Pille Pärn - Chair of Animal Breeding and Biotechnology, Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, Kreutzwaldi 62, 51014 Tartu, Estonia Department of Pathophysiology, Institute of Biomedicine and Translational Medicine, University of Tartu, 50411 Tartu, Estonia Competence Centre on Health Technologies, 50411 Tartu, Estonia Estonian Genome Centre, Institute of Genomics, University of Tartu, 51010 Tartu, Estonia Centre for Molecular Medicine and Innovative Therapeutics, Murdoch University, Perth, WA 6150, Australia Perron Institute for Neurological and Translational Science, Perth, WA 6009, Australia
Ene Reimann - University of Tartu
Sulev Kõks - Murdoch University, Centre for Molecular Medicine and Innovative Therapeutics
Ülle Jaakma - Chair of Animal Breeding and Biotechnology, Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, Kreutzwaldi 62, 51014 Tartu, Estonia Department of Pathophysiology, Institute of Biomedicine and Translational Medicine, University of Tartu, 50411 Tartu, Estonia Competence Centre on Health Technologies, 50411 Tartu, Estonia Estonian Genome Centre, Institute of Genomics, University of Tartu, 51010 Tartu, Estonia Centre for Molecular Medicine and Innovative Therapeutics, Murdoch University, Perth, WA 6150, Australia Perron Institute for Neurological and Translational Science, Perth, WA 6009, Australia
Publication Details: Genes, Vol.14(3), Art. 569
Publisher: MDPI
Grant note: IUT 8-1 / Institutional research funding EU30020 / Enterprise Estonia 692299 / Horizon 2020 Project SEARMET PRG1665 / Estonian Research Council
Identifiers: 991005565368707891
Murdoch Affiliation: Centre for Molecular Medicine and Innovative Therapeutics
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.81 Reproductive Biology; 1.81.339 Embryo Development
Web Of Science research areas: Genetics & Heredity
ESI research areas: Molecular Biology & Genetics